Methods Preparation Electron Microscopy (26 results)

Language: English
Published by Berlin ; Heidelberg ; New York ; London ; Paris ; Tokyo : Springer, 1987
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Condition: Gut. XVII, 190 S. : 62 Ill., graph. Darst. Gebraucht; Einband minimal berieben, ansonten guter Zustand. Aus dem Inhalt: An Introduction to Electron Microscops (EM) / Methods for TEM / Methods for SEM / Evaluation of Micrographs / Appendix: Buffers in Electron Microscopy / Subject Index. Sprache: Englisch Gewicht in Gr…amm: 350.

Language: English
Published by New York, American Elsevier Publishing, 1985
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- First Edition
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8° , Hardcover/Pappeinband. 1.Auflage.. XIII, 206 Seiten mit einigen Abbildungen, Einband etwas berieben, Bibl.Ex., ansonst guter und sauberer Zustand 0444807012 Sprache: Englisch Gewicht in Gramm: 560.

Methods of Preparation for Electron Microscopy
Robinson, David G.; Ehlers, Ulrich; Herken, Rainer; Herrmann, Bernd; Mayer, Frank; Schürmann, Friedrich-Wilhelm
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Methods of Preparation for Electron Microscopy
David G. Robinson|Ulrich Ehlers|Rainer Herken|Bernd Herrmann|Frank Mayer|Friedrich-Wilhelm Schürmann
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Language: English
Published by North-Holland Publishing / American Elsevier, 1972
- Hardcover
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the…light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Language: English
Published by CRC Press, 2008
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Language: English
Published by CRC Press 2008-10-02, 2008
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Handbook of Cryo-Preparation Methods for Electron Microscopy
Cavalier, Annie (EDT); Spehner, Daniele (EDT); Humbel, Bruno M. (EDT)
Language: English
Published by CRC Press, 2008
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Handbook of Cryo-Preparation Methods for Electron Microscopy
Cavalier, Annie (EDT); Spehner, Daniele (EDT); Humbel, Bruno M. (EDT)
Language: English
Published by CRC Press, 2008
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Handbook of Cryo-Preparation Methods for Electron Microscopy
Cavalier, Annie (EDT); Spehner, Daniele (EDT); Humbel, Bruno M. (EDT)
Language: English
Published by CRC Press, 2008
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Handbook of Cryo-Preparation Methods for Electron Microscopy
Cavalier, Annie (EDT); Spehner, Daniele (EDT); Humbel, Bruno M. (EDT)
Language: English
Published by CRC Press, 2008
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Language: English
Published by Taylor & Francis Group, 2008
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Condition: New. pp. 706.

Language: English
Published by CRC Press, 2008
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Language: English
Published by CRC Press, 2008
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Language: English
Published by Taylor & Francis Group, 2008
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Handbook of Cryo-Preparation Methods for Electron Microscopy
Cavalier, Annie (Edited by)/ Spehner, Daniele (Edited by)/ Humbel, Bruno M. (Edited by)
Language: English
Published by CRC Pr I Llc, 2008
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Hardcover. Condition: Brand New. 1st edition. 704 pages. 10.28x7.17x1.34 inches. In Stock.

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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the res…o lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus. 212 pp. Englisch.

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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lu…tion of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 212 pp. Englisch.

Language: English
Published by Taylor & Francis Group, 2008
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Language: English
Published by CRC Press, 2008
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Language: English
Published by CRC Press, 2008
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Language: English
Published by CRC Press, 2008
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Buch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to pres…ent the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.