Handbook of Cryo-Preparation Methods for Electron Microscopy
Language: English
Published by CRC Press, 2008
- Hardcover
- New
Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH
AbeBooks seller since August 14, 2006
Condition: New
£ 508.49
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nach der Bestellung gedruckt Neuware - Printed after ordering - Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.
Seller Inventory # 9780849372278
- Title
- Handbook of Cryo-Preparation Methods for Electron Microscopy
- Author
- Bruno M. Humbel
- Publisher
- CRC Press
- Publication year
- 2008
- Condition
- Neu
- Binding
- Buch
- Language
- English
- ISBN 10
- 0849372275
- ISBN 13
- 9780849372278
- Item weight
- 1,500 grams
- Dimensions
- 260x183x42 mm
- Series
- Book 5 of 6: Methods in Visualization
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.
The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.
Contributions to this handbook –
Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis
Ö Teach how to arrest physiological processes by cryo-fixation
Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS
Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography
Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules
Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography
Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding
Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling
Ö Include summary tables to compare and obtain appropriate criteria
Includes a wealth of immediate reference material for daily use
With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.
"Synopsis" may belong to another edition of this title.
About the Author
"About the title" may belong to another edition of this title.
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