Methods Protein Nucleic Acid by Osterman (33 results)
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - A consideration of the different types of immunoelectrophoresis is the natural con clusion to the methods of electrophoretic fractionation described in the preceding book for proteins and nucleic acids. Immunoprecipitation is the essential com pone…nt process of immunoelectrophoresis, and is in itself a highly specific method of purifying proteins. It can also be used for their detection after conventional elec trophoresis or isoelectrofocusing. It is therefore worthwhile considering the mecha nism and specific features of immunoprecipitation before considering im munoelectrophoresis itself. Some of these features, in particular, the ambiguity of the immune response and the polymorphism ofimmune reactions, cannot be under stood without abrief but not superficial knowledge of the mechanism of generating immunity, the structure and function of the immunoglobulins, and the nature of antigen-antibody interactions. A knowledge of these topics is necessary for an understanding of the radioimmune methods dealt with in the second Part of the book. In the majority of cases biochemists and molecular biologists are inadequately acquainted with contemporary immunochemistry, which is currently undergoing rapid development. All this justifies the inclusion of a detailed introduction to immunochemistry as the first chapter. Chapter 2 is devoted to adescription of the use of antisera in the immunological assay of components separated by conventional electrophoresis and IEF. The remaining three Chapters analyze the different forms of immuno electrophoresis, usually carried out in agarose gels.
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikel…y to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.
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Language: English
Published by Springer Berlin Heidelberg, Springer Apr 2012, 2012
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A consideration of the different types of immunoelectrophoresis is the natural con clusion to the methods of electrophoretic fractionation described in the preceding book for proteins and nucleic acids. Immunoprecipitation is the es…sential com ponent process of immunoelectrophoresis, and is in itself a highly specific method of purifying proteins. It can also be used for their detection after conventional elec trophoresis or isoelectrofocusing. It is therefore worthwhile considering the mecha nism and specific features of immunoprecipitation before considering im munoelectrophoresis itself. Some of these features, in particular, the ambiguity of the immune response and the polymorphism ofimmune reactions, cannot be under stood without abrief but not superficial knowledge of the mechanism of generating immunity, the structure and function of the immunoglobulins, and the nature of antigen-antibody interactions. A knowledge of these topics is necessary for an understanding of the radioimmune methods dealt with in the second Part of the book. In the majority of cases biochemists and molecular biologists are inadequately acquainted with contemporary immunochemistry, which is currently undergoing rapid development. All this justifies the inclusion of a detailed introduction to immunochemistry as the first chapter. Chapter 2 is devoted to adescription of the use of antisera in the immunological assay of components separated by conventional electrophoresis and IEF. The remaining three Chapters analyze the different forms of immuno electrophoresis, usually carried out in agarose gels. 220 pp. Englisch.
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. I Immunoelectrophoresis.- 1 Fundamentals of Immunochemistry.- 1.1 The Immune System of Higher Organisms.- 1.1.1 Blood Components.- 1.1.2 Phagocytosis, Complement, Cytointoxication.- 1.1.3 Leucocytes.- 1.1.4 Antibodies….- 1.1.5 The Lymphatic System and the Sp.
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -I Immunoelectrophoresis.- 1 Fundamentals of Immunochemistry.- 2 The Use of Immune Methods to Detect Protein Zones Following Conventional Electrophoresis and Electrofocusing.- 3 Immunoelectrophoresis According to Grabar and Williams.- 4…Laurell Rocket Immunoelectrophoresis.- 5 Crossed Immunoelectrophoresis.- II The Use of Radioisotopes.- 1 Isotopes, Scintillators, and Scintillation Counters.- 2 Preparation and Counting of Labelled Samples.- 3 Labelling of the Proteins.- 4 Labelling of Nucleic Acids.- 5 Labelling of Proteins and Nucleic Acids in Vivo.- 6 Determination of Concentrations by Isotope Dilution.- 7 Radioimmune Methods.- Some Additional Remarks on the Use of Radioactive Isotopes.- References to Part I.- References to Part II.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 220 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step… is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity. 356 pp. Englisch.
