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Published by CRC Press 2008-10-02, 2008
ISBN 10: 0849372275 ISBN 13: 9780849372278
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Published by Taylor & Francis Group, 2008
ISBN 10: 0849372275 ISBN 13: 9780849372278
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Published by Taylor & Francis Group, 2008
ISBN 10: 0849372275 ISBN 13: 9780849372278
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Published by Taylor & Francis Group, 2008
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Buch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.Contributions to this handbookÖ Describe cryo-methods aimed at perfect preservation for fine structural analysisÖ Teach how to arrest physiological processes by cryo-fixationÖ Reveal the secrets for high-resolution snapshots of life by CEMOVISÖ Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomographyÖ Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and moleculesÖ Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geographyÖ Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embeddingÖ Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabelingÖ Include summary tables to compare and obtain appropriate criteriaIncludes a wealth of immediate reference material for daily useWith clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.