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Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
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Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
Seller: Books Puddle, New York, NY, U.S.A.Books Puddle
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Condition: New. pp. 80.
Language: English
Published by Springer Verlag, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
Seller: Revaluation Books, Exeter, United KingdomRevaluation Books
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Paperback. Condition: Brand New. 1st edition. 65 pages. 8.75x6.00x0.25 inches. In Stock.
Language: English
Published by Springer New York, Springer US, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot…analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is appliedto proteins.
Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
Seller: Mispah books, Redhill, SURRE, United KingdomMispah books
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Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
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Language: English
Published by Springer New York Sep 2013, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
- Print on Demand
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermanyBuchWeltWeit Ludwig Meier e.K.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is t…he Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins. 80 pp. Englisch.
Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
- Print on Demand
Seller: Majestic Books, Hounslow, United KingdomMajestic Books
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Condition: New. Print on Demand pp. 80 14 Illus. (11 Col.).
Language: English
Published by Springer, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
- Print on Demand
Seller: Biblios, frankfurt am main, HESSE, GermanyBiblios
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Condition: New. PRINT ON DEMAND pp. 80.
Language: English
Published by Springer New York, 2013
Series: SpringerBriefs in Systems Biology, Book 5 of 12. Book 5 of 12 - SpringerBriefs in Systems Biology
- Softcover
- Print on Demand
Seller: moluna, Greven, Germanymoluna
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Kartoniert / Broschiert. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Explains both the theoretical and experimental details of the selected reaction monitoring experiment as it is applied to proteinsDescribes a new type of quantitative proteomics experiment cal…led selected reaction monitoringReviews the expe.
