Surface Plasmon Resonance Methods (34 results)
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Condition: Good. Your purchase helps support Sri Lankan Children's Charity 'The Rainbow Centre'. Ex-library, so some stamps and wear, but in good overall condition. Our donations to The Rainbow Centre have helped provide an education and a safe haven to hundreds of children who live in appalling conditions.
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Condition: New. 2016. Softcover reprint of the original 1st ed. 2010. paperback. . . . . .
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Taschenbuch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, whil…e the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interactionof proteins using SPR.
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Taschenbuch. Condition: Neu. Surface Plasmon Resonance | Methods and Protocols | Nico J. De Mol (u. a.) | Taschenbuch | x | Englisch | 2016 | Humana | EAN 9781493961252 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]…com | Anbieter: preigu.
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Taschenbuch. Condition: Neu. Methods for Fragments Screening Using Surface Plasmon Resonance | Sameer Mahmood Zaheer (u. a.) | Taschenbuch | xvi | Englisch | 2022 | Springer | EAN 9789811615382 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com… | Anbieter: preigu.
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Condition: New. 2016. Softcover reprint of the original 1st ed. 2010. paperback. . . . . . Books ship from the US and Ireland.
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Condition: Sehr gut. Zustand: Sehr gut | Seiten: 296 | Sprache: Englisch | Produktart: Bücher | Surface plasmon resonance (SPR) has evolved into an exciting technique in biomolecular interaction analysis. The development of commercial SPR instruments has made the te- nique available to a wide scienti?c audience, and the number o…f publications in which the use of SPR is described is rapidly increasing. SPR is in use for many purposes from food quality control to the study of nanoparticles. Much research is now focused on devel- ing new SPR-related applications, e.g., SPR imaging, SPR arrays, SPR ?uorescence, and combinations of SPR with mass spectrometry and with electrochemistry. Biomolecular interaction analysis is at the core of many research projects. In principle, the setup of an SPR experiment is simple: There is a sensor surface to which one of the interacting partners (the ligand) is immobilized; the other partner (the analyte) is added in a ?ow or cell-like compartment. The binding phenomenon is monitored in real time as a change in SPR angle. An important issue is the choice of surface and the immobilization strategy. With SPR, it is possible to mimic the biological environment which is relevant for an interaction. For interactions in a water environment, sensor surfaces with hydrogels are available. Many biomolecular interactions take place in a membrane environment. For this, commercial sensor surfaces are available, or surfaces can be tailor-made. This volume contains several examples of building up of lipophilic surfaces. Nature abundantly makes use of multivalent interactions; multivalency can be mimicked on a sensor surface with immobilized ligands.
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Buch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the s…ubsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interactionof proteins using SPR.
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Hardcover. Condition: Brand New. 1st edition. 286 pages. 10.00x7.00x0.75 inches. In Stock.
Methods for fragments screening using Surface Plasmon Resonance
Zaheer, Sameer Mahmood (Edited by)/ Gosu, Ramachandraiah (Edited by)
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Hardcover. Condition: Brand New. 129 pages. 10.00x7.01x0.38 inches. In Stock.
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paperback. Condition: New. Paperback.Pub Date:2023-08 Pages:191 Language: Chinese Publisher: China University of Geosciences Press Sensor technology is a rapidly developing high-tech that is remarkable in the world today. It is also an important symbol of the development of contemporary science and technology. It is closely rela…ted to Communication technology and computer technology together constitute the three pillars of the information industry. Sensors are an important technical foundation for the information socie.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular in…teractions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR. 132 pp. Englisch.
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Discusses real-time and label-free measurement of biomolecular interactionPresent protocols to calculate kinetics and affinities with single analyte concentrationExamines techniques for the optimum amine and biotin-st…reptavidin coupling.
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signa…l. In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments.The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.
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Condition: New. PRINT ON DEMAND pp. 296.
Language: English
Published by Springer Nature Singapore, Springer Nature Singapore Sep 2022, 2022
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular intera…ctions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interactionof proteins using SPR.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 132 pp. Englisch.
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Gebunden. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Discusses real-time and label-free measurement of biomolecular interactionPresent protocols to calculate kinetics and affinities with single analyte concentrationExamines techniques for the optimum amine and… biotin-streptavidin coupling.
