Isbn: 9783639098921 - Opto-injection Into Single Living Cells by Femtosecond Nir Laser: a Novel Technique of Delivering Materials Into Liveing Cells (14 results)
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Published by VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2008
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Published by VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2008
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Published by VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2008
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This dissertation presents a novel technique ofdelivering membrane impermeable molecules into singleliving cells with the assistance of femtosecond (fs)near-infrared (NIR) laser pulses. This techniquepromises several major advantages, namely, very hightransfection efficiency, high cell survival rate( 100%) and fully preserved cell viabilities. In this work, the system for fs NIR opto-injectionwas designed and built. Successful fs NIRopto-injection has been performed on several cellsystems including single mammalian cells, marineanimal eggs, and human cancer cells cultured in atissue-like environment. The connections betweenlaser parameters and cell responses were explored.Dye uptake rate of the target cells was observed todepend on incident laser intensity. Pore size wasfound dependent on incident laser intensity. Theconclusion was made that laser-induced breakdown andplasma-induced ablation in cell membrane are thephysical principles that govern the process of fs NIRopto-injection.…
