Protocols For Nucleic Acid Analysis By Nonradioactive Probes (Pb)

Isaac P.G.

ISBN 10: 089603254X / ISBN 13: 9780896032545

Published by Humana Press, Totowa, N.J, 1994

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Bibliographic Details

• Title: Protocols For Nucleic Acid Analysis By Nonradioactive Probes (Pb)
• Publisher: Humana Press, Totowa, N.J
• Publication Date: 1994
• Language: English
• ISBN 10: 089603254X
• ISBN 13: 9780896032545
• Binding: Soft cover
• Condition: Brand New

About this title

Synopsis

This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

Review

...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters
...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry