Expression, Purification and Characterization of recombinant Xylanases

Language: English

Published by LAP LAMBERT Academic Publishing Aug 2018, 2018

6139899230 / 9786139899234

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This item is printed on demand - Print on Demand Titel. Neuware -The book aims to compare some of the kinetic properties of covalently immobilized xylanase on the surface of glutaraldehyde activated alginate beads with free xylanase. Immobilization is defined as the imprisonment of cell or enzyme in a distinct support or matrix. The support or matrix on which the enzymes are immobilized allows the exchange of medium containing substrate or effector or inhibitor molecules. The practice of immobilization of cells is very old and the first immobilized enzyme was amino acylase of Aspergillus oryzae for the production of L-amino acids in Japan. The immobilized enzyme displayed higher Vmax, Km and kcat values but lower kcat/Km value in comparison to its free counterpart. Overall, the 'catalytic efficiency' of xylanase was found to decline with immobilization. The isokinetic temperature (Tisokin) of free and immobilized xylanase was recorded at 86.5ºC with ln kd value of about -4.0. The method of immobilization provided 'low temperature stabilization' effect to xylanase which makes it more thermostable at temperatures lower than the Tisokin.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 84 pp. Englisch.…

Seller Inventory # 9786139899234

Title
Expression, Purification and Characterization of recombinant Xylanases
Author
Hetal Patel
Publisher
LAP LAMBERT Academic Publishing Aug 2018
Publication year
2018
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
6139899230
ISBN 13
9786139899234
Item weight
143 grams
Dimensions
220x150x6 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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