Expression, Purification and Characterization of recombinant Xylanases - Softcover

Patel, Hetal; Panchal, Malti; Patel, Niyati

 
9786139899234: Expression, Purification and Characterization of recombinant Xylanases
Softcover
ISBN 10: 6139899230 ISBN 13: 9786139899234
Publisher: LAP LAMBERT Academic Publishing, 2018

Synopsis

The book aims to compare some of the kinetic properties of covalently immobilized xylanase on the surface of glutaraldehyde activated alginate beads with free xylanase. Immobilization is defined as the imprisonment of cell or enzyme in a distinct support or matrix. The support or matrix on which the enzymes are immobilized allows the exchange of medium containing substrate or effector or inhibitor molecules. The practice of immobilization of cells is very old and the first immobilized enzyme was amino acylase of Aspergillus oryzae for the production of L-amino acids in Japan. The immobilized enzyme displayed higher Vmax, Km and kcat values but lower kcat/Km value in comparison to its free counterpart. Overall, the ‘catalytic efficiency’ of xylanase was found to decline with immobilization. The isokinetic temperature (Tisokin) of free and immobilized xylanase was recorded at 86.5ºC with ln kd value of about -4.0. The method of immobilization provided ‘low temperature stabilization’ effect to xylanase which makes it more thermostable at temperatures lower than the Tisokin.

"synopsis" may belong to another edition of this title.

Publisher
LAP LAMBERT Academic Publishing
Publication date
2018
Language
English
ISBN 10 
6139899230
ISBN 13 
9786139899234
Binding
Paperback
Number of pages
84