Mass Spectrometry in the Biological Sciences: A Tutorial: 353 (Nato Science Series C:, 353) - Hardcover

Hardcover

ISBN 10: 0792315391 ISBN 13: 9780792315391

Publisher: Kluwer Academic Publishers, 1991

Synopsis

The developments in mass spectrometry over the past fifteen years have been impressive in their implications in bioanalytical chemistry. The achievements begin with the inventions of Cf-252 Plasma Desorption Mass Spectrometry by Macfarlane and Fourier Transform Mass Spectrometry by Comisarow and Marshall in the mid 1970s. The former showed the feasibility of producing large gas-phase ions from large biomolecules whereas the latter enhanced the capabilities for ion trapping especially in analytical mass spectrometry. A major achievement was the development by Barber of Fast Atom Bombardment (FAB) mass spectrometry, an advance that heralded a new era in biological mass spectrometry. Contemporary and routine instruments such as magnetic sectors and quadrupoles were rapidly adapted to F AB, and nearly the entire universe of small molecules became amenable to study by mass spectrometry. The introduction of FAB also paved the way for improvement of instrument capability. For example, the upper mass limit of magnet sector mass spectrometers was increased by nearly an order of magnitude by the instrument manufacturers. Furthermore, the technique of tandem mass spectrometry (MS/MS) was given new meaning because important structural information for biomolecules could now be produced for ions introduced by FAB into the tandem instrument. The evolution of MS/MS continues today with the development of ion traps, time-of-flight, and sector instruments equipped with array detection.

About this title

Review

`I therefore can be highly recommended to all chemists and physicist who are interested to learn about the current applicability of mass spectrometry to biological sciences and in particular to those who are practising this area of research.'
Recueil dex Travaux Chimiques des Pays-Bas
'In conclusion, Mass Spectrometry in the sciences: A Tutorial achieves its objective as a tutorial for biological mass spectrometry. This book would be an excellent complement to an introductory mass spectrometry book because it contains the most recent techniques...covered by most introductory texts....I recommend this book as an ideal primer to understanding the current status of biological mass spectrometry' 'Applied Spectroscopy' 46:10 1992

Synopsis

This book covers both the basic principles of contemporary mass spectrometry instrumentation as well as its application to problems in structural biochemistry. Its tutorial form serves as an introduction to those entering the field, and for established scientists who wish to apply mass spectrometry to structural problems. The expertly written chapters introduce and summarize the capabilities of sector, tandem sector, hybrid, time-of-flight, triple quadrupole, ion trap, and Fourier transform mass spectrometers. New methods, such as electrospray ionization, laser desorption, and high performance liquid chromatography are also covered. Additional chapters report case studies and approaches to the determination of peptides, nucleosides, nucleotides, and lipids.

Edition details

• Publisher: Kluwer Academic Publishers
• Publication date: 1991
• Language: English
• ISBN 10: 0792315391
• ISBN 13: 9780792315391
• Binding: Hardcover
• Number of pages: 482
• Editor: Gross M.L