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Applications of Continuous and Steady-State Methods to Root Biology: 34 (Developments in Plant and Soil Sciences, 34) - Hardcover
This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.
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` This book deserves to be purchased by every library and institute as the most uptodate reference work on continuous and steady-state methods and their application to root biology. '
A. Ekbote, Indian Journal of Agricultural Sciences, Vol. 60, no. 4
A. Ekbote, Indian Journal of Agricultural Sciences, Vol. 60, no. 4
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- PublisherSpringer
- Publication date1989
- ISBN 10 0792300246
- ISBN 13 9780792300243
- BindingHardcover
- Number of pages264
- EditorTorrey John G., Winship Lawrence J.
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Applications of Continuous and Steady-State Methods to Root Biology
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Applications of Continuous and Steady-State Methods to Root Biology (Developments in Plant and Soil Sciences, 34)
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Applications of Continuous and Steady-State Methods to Root Biology
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Book Description Buch. Condition: Neu. Druck auf Anfrage Neuware - Printed after ordering - This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes. Seller Inventory # 9780792300243
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Applications of Continuous and Steady-State Methods to Root Biology
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Book Description Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes. 268 pp. Englisch. Seller Inventory # 9780792300243
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