Chromosome Structural Analysis: A Practical Approach: No. 200 - Hardcover

Bickmore, Wendy A.

Hardcover

ISBN 10: 0199636990 ISBN 13: 9780199636990

Publisher: Oxford University Press, 1999

Synopsis

The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (for example, histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein: DNA interactions "in vivo" using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei "in vitro". protein, DNA interactions and genetic manipulation This bo

About this title

Review

"...The book is an essential read for all workers in fields such as molecular biology, genetics and biomedical science whose work involves chromosome analysis." Clinical Laboratory International, September 1999

About the Author

Dr Wendy A. Bickmore, Cell Genetics Section, MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU. Tel: 0131 332 2471. Fax: 0131 343 2620. Email: wendy@hgu.mrc.ac.uk

Edition details

• Publisher: Oxford University Press
• Publication date: 1999
• Language: English
• ISBN 10: 0199636990
• ISBN 13: 9780199636990
• Binding: Hardcover
• Number of pages: 209