RNA-Protein Interactions: A Practical Approach: No.192 - Hardcover

Smith, Christopher B. R.

Hardcover

ISBN 10: 0199636516 ISBN 13: 9780199636518

Publisher: Oxford University Press, 1998

Synopsis

RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly-catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. This text provides a guide to the experimental procedures used in studying RNA-protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in-depth analysis of the structural basis of the interaction.

About this title

Review

"There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro
transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental
protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover."--The
Quarterly Review of Biology
"There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro
transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental
protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover."--The
Quarterly Review of Biology
"There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover."--The Quarterly Review of Biology
"There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover."--The Quarterly Review of Biology

About the Author

Dr Christopher W J Smith, Lecturer in Biochemistry, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Old Addenbrooke's Site, Cambridge CB2 1GA. Tel: 01223 333655 / 333665. Fax: 01223 766002. Email: cwjs1@mole.bio.cam.ac.uk

Edition details

• Publisher: Oxford University Press
• Publication date: 1998
• Language: English
• ISBN 10: 0199636516
• ISBN 13: 9780199636518
• Binding: Hardcover
• Number of pages: 320