Growth Factors and Receptors: A Practical Approach: No.194 - Hardcover

McKay, Ian

Hardcover

ISBN 10: 0199636478 ISBN 13: 9780199636471

Publisher: Oxford University Press, 1998

Synopsis

Provides comprehensive protocols for studies of growth factors and their interactions with receptors. It covers a wide range, from simple analytical techniques to sophisticated in vivo applications including: RT-PCR and immunocytochemistry for detection of growth factors and receptors; production and purification of recombinant growth factors and receptors; labelling of growth factors for binding studies; in vivo mutagenesis; the yeast two-hybrid assay of protein-protein interactions; phage display of factors; application of factors to wound-healing processes using the gene gun; treatment of cancers with factor/toxin chimeras; and analysis of important factor domains using chimeric proteins. This book updates and extends the current literature and describes important novel approaches to the study of growth factors and their receptors, including the use of RNA aptamers as receptor antagonists, and the development of receptor superantagonists.

About this title

About the Author

Dr Ian A. McKay Senior Lecturer in Dermatology Centre for Cutaneous Research 2 Newark Street London E1 2AT Tel: 0171 295 7160 Fax: 0171 295 7171 Email: I.A.McKay@mds.qmw.ac.uk Dr Kenneth D. Brown Institute of Animal Physiology The Babraham Institute Babraham Hall Babraham, Cambridge CB2 4AT Tel: 01223 833312 Fax: 01223 833511 Email: kbrown@bbsrc.ac.uk

Edition details

• Publisher: Oxford University Press
• Publication date: 1998
• Language: English
• ISBN 10: 0199636478
• ISBN 13: 9780199636471
• Binding: Hardcover
• Number of pages: 256