Items related to PCR: PCR in Situ Hybridization v.3 (A Practical Approach)
Synopsis
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniques in situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book is intended for graduates and post-doctoral researchers, academic staff, and clinicians across a wide range of molecular medical disciplines.
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Review
"...the book gives an excellent overview of the practical application of PCRIS." Molecular Pathology, 1999
"This is one of an impressive series of laboratory handbooks entitled The Practical Approach Series...The presentation of this material is of uniformly high quality...this book would be a valuable resource in a lab" Canadian Journal of Medical Laboratory Scince
About the Author
C. S. Herrington is at University of Liverpool. J. J. O'Leary is at John Radcliffe Hospital, Oxford.
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Search results for PCR: PCR in Situ Hybridization v.3 (A Practical Approach)
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Pcr 3: Pcr in Situ Hybridization : A Practical Approach (Practical Approach Series)
Published by
Oxford University Press, 1998
ISBN 10: 0199636338
ISBN 13: 9780199636334
Used
Hardcover
Seller: medimops, Berlin, Germany
Seller rating 5 out of 5 stars
Condition: very good. Gut/Very good: Buch bzw. Schutzumschlag mit wenigen Gebrauchsspuren an Einband, Schutzumschlag oder Seiten. / Describes a book or dust jacket that does show some signs of wear on either the binding, dust jacket or pages. Seller Inventory # M00199636338-V
Quantity: 1 available
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PCR 3: PCR In Situ Hybridization - A Practical Approach
Published by
IRL Press at Oxford University Press, Oxford, New York, and Tokyo, 1998
ISBN 10: 0199636338
ISBN 13: 9780199636334
Used
Hardcover
First Edition
Seller: Vashon Island Books, Vashon, WA, U.S.A.
Seller rating 5 out of 5 stars
Hardcover. Condition: Near Fine. No Jacket. First Edition. Size: 8vo - over 7¾" - 9¾" tall. Book. Seller Inventory # G8557
Quantity: 1 available