Platelets: A Practical Approach: No.167 - Hardcover

Watson, Stephen

Hardcover

ISBN 10: 0199635382 ISBN 13: 9780199635382

Publisher: Oxford University Press, 1996

Synopsis

Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. This volume describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest methodology, such as monitoring calcium levels in single cells, protein tyrosine phosphorylation and PCR.

About this title

Review

recent addition to the well known and much respected Practical Approach Series. ... In common with the other volumes in the series, the book provides detailed and comprehensive laboratory protocols for the study of all aspects of platelet function. ... This is not a book for the merely curious, however. it is aimed squarely at those with a determined research interest in platelet function or signal transduction. ... The clear and crisp descriptions are sufficient to allow a comparative novice in the field to establish the methods with relative ease. ... Platelets should prove useful to anyone with an interest in the biochemistry of cellular signal transduction. (British Journal of Biomedical Science 1997)

From the Back Cover

Platelets play an essential role in blood clotting at sites of wound injury, but unwanted activation of platelets in the circulation can give rise to thrombus formation, and is implicated in the onset of stroke, myocardial infarction, and other diseases. Platelets are also one of the major model systems for the study of signal transduction. This book is a comprehensive and up-to-date practical guide to research on platelets. Each chapter, written by experts in the field, contains detailed protocols, illustrative results, tips and hints for success, and key literature citations. Topics covered include: platelet preparation; monitoring platelet aggregation and secretion; megakaryocyte differentiation; preparation and uses of semipermeabilized platelets; monitoring calcium; identification and characterization of platelet glycoproteins (including the use of PCR); analysis of cell-surface antigens; the role of the cytoskeleton in platelet function; measurement of protein serine/threonine and tyrosine phosphorylation, kinase activity, cyclic nucleotides and G protein function; analysis of phospholipid metabolism, immunocytochemistry and electron microscopy studies; clinical aspects of platelet function; platelet procoagulant activity; and studies of platelet function in species other than man.

Edition details

• Publisher: Oxford University Press
• Publication date: 1996
• Language: English
• ISBN 10: 0199635382
• ISBN 13: 9780199635382
• Binding: Hardcover
• Number of pages: 396