HIV: Biochemistry, Molecular Biology and Drug Discovery v.2 (A Practical Approach) - Hardcover

Karn, Jonathan

Hardcover

ISBN 10: 0199634998 ISBN 13: 9780199634996

Publisher: Oxford University Press, 1996

Synopsis

HIV Volume 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described as well as assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery. Contents: The HIV life cycle; Reverse transcriptase; Ribonulease H; Integrase; Protease; Expression of gag, envelope and other HIV antigens using Bacculovirus; Envelope expression and purification; HIV envelope glyocoprotein/CD4 interactions - studies using recombinant vaccinia virus vectors; RNA binding assays for the regulatory proteins tat and rev; Cellular and cell-free assays for tat; Cellular assays for rev; DNA binding proteins interacting with the viral LTR; Nef; Vpu; Vif; Cellular assays for antiviral drugs; Inhibition of HIV by ribozymes and antisense oligonucleotides; Gene therapy approaches to the inhibition of HIV This book is intended for these books provide instructions in virological, biochemical, molecular biological, and immunological methods of use for HIV researchers in academic institutions, medical schools, and the pharmaceutical industry.

About this title

Review

HIV: a Practical Approach is the latest two-volume offering from the Practical Approach series and provides the first comprehensive methodology for the HIV field. As such, it can be recommended strongly to all active bench scientists in the field as well as to desk-bound research directors who wish to keep in touch ... well worth the effort. (James C. Neil, University of Glasgow Veterinary School, Molecular Medicine Today, October 1996)

The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interaction between cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratory working with HIV. (M.A. Koch, Zentralblatt fur Bakteriologie 1996)

About the Author

J. Karn is at MRC Laboratory of Molecular Biology, Cambridge.

Edition details

• Publisher: Oxford University Press
• Publication date: 1996
• Language: English
• ISBN 10: 0199634998
• ISBN 13: 9780199634996
• Binding: Hardcover
• Number of pages: 353