Bioenergetics: A Practical Approach: No.154 - Hardcover

Brown, G. C.

9780199634897: Bioenergetics: A Practical Approach: No.154

Hardcover

ISBN 10: 0199634890 ISBN 13: 9780199634897

Publisher: Oxford University Press, 1995

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Bioenergetics is the study of energy in living organisms and is a central topic of modern biology. The movement of electrons and ions across and through membranes is important in fields such as energy transduction, cell biology, and cell signalling. Current research in bioenergetics impinges on a wide range of fundamental problems in biology and scientists in other areas have had to learn classical bioenergetic techniques. Written by international experts in the field, this handbook describes all the major techniques and methods of analysis in a practical and accessible format. Contents: Oxygen, proton, and phosphate fluxes and stoichiometries; Membrane permeability and transport; The protonmotive force; Reconstitution of bioenergetic proteins and the uses of proteoliposomes; Redox states and potentials; Control and regulation in bioenergetics; Patch clamping of bioenergetic membranes and proteoliposomes; Studies of cellular energetics using 31P-NMR; Isolation and characterization of photosynthetic reaction centres from eukaryotic organisms. This book is intended for research scientists working in the areas of mammalian biochemistry, plant biochemistry, and cell biology, studying bioenergetics.

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The movement of electrons, protons, and other ions across and through membranes is crucially important in many fields including energy transduction, cell biology, and cell signalling. This book brings together the key methods and approaches of modern bioenergetics, to assist the researcher to probe and understand these phenomena. Topics covered include: measurement of oxygen, proton, and phosphate fluxes and stoichiometries; membrane permeability and transport; reconstitution of bioenergetic proteins and use of proteoliposomes; protonmotive force; redox states and potentials; control and regulation; patch clamping; studies of cellular energetics using (superscript 31)P-NMR; isolation and characterization of photosynthetic reaction centres in eukaryotes. Bioenergetics: A Practical Approach will be invaluable to established researchers as well as those who need to use these techniques for the first time. It is a comprehensive practical manual providing not only up-to-date laboratory protocols, but also many hints and tips for success, troubleshooting comments, and the essential theory behind important experimental approaches.