Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of proteins of commercial interest. Part of a four-volume set on DNA cloning, this book covers the main expression systems in current use. It provides background information and advice on the merits of each, step-by-step practical protocols, and details of the latest applications.
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a worthwhile bench top companion (Henry Brzeski, University of Strathclyde, Biochemical Systematics and Ecology, Vol. 24, No. 1, 1996)
The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in the area of heterologous protein expression ... The book can thus be highly recommended. (Bent Honoré, FEBS Letters 393 (1996))
B. D. Hames is at University of Leeds. D. Glover is at University of Dundee.
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