RNA Processing: v.2: A Practical Approach - Hardcover

Higgins, Stephen J.

Hardcover

ISBN 10: 0199634718 ISBN 13: 9780199634712

Publisher: Oxford University Press, 1994

Synopsis

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full desciptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.

Edition details

• Publisher: Oxford University Press
• Publication date: 1994
• Language: English
• ISBN 10: 0199634718
• ISBN 13: 9780199634712
• Binding: Hardcover
• Number of pages: 278