Plasmids: A Practical Approach: No. 138 - Hardcover

Hardcover

ISBN 10: 0199634459 ISBN 13: 9780199634453

Publisher: Oxford University Press, 1994

Synopsis

This work is designed to provide research workers with detailed protocols for studying bacterial plasmids, and for using both plasmids and phagemids as vectors. The techniques for studying plasmid replication and maintenance are described in detail, as are fundamental methods for purifying plasmids and for introducing them into cells. Also included are protocols for mutagenesis, and for analyzing plasmid-encoded products. Other chapters focus on techniques for studying plasmids and for using plasmid vectors in particularly important groups of bacteria - animal and plant pathogens harbouring virulence plasmids, streptomyces and lactococci. Plasmid composite vectors and phagemids of various kinds are useful for expression, cDNA cloning and other genetic manipulations. The protocols for using them, as well as important yeast shuttle vectors are described in detail.

About this title

Review

'this volume provides practical guidance on plasmid replication and maintenance, methods for purifying plasmids and introducing them into cells ... essential reading for all those using recombinant DNA techniques'Aslib Book Guide, Vol. 59, No. 4, April 1994

The book will be essential reading for all those using recombinant DNA techniques. (Aslib Book Guide, Vol. 59, No. 4, April 1994)

About the Author

K. G. Hardy, Assistant Director, Glaxo Institute for Molecular Biology, Geneva.

Edition details

• Publisher: Oxford University Press
• Publication date: 1994
• Language: English
• ISBN 10: 0199634459
• ISBN 13: 9780199634453
• Binding: Hardcover
• Edition number: 2
• Number of pages: 270
• Editor: Hardy K. G.