Synopsis
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end processing, capping, methylation, splicing and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all key steps required for successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNA's and the isolation and analysis of ribonucleoprotein complexes.
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Rna Processing, Vol. 1
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RNA Processing
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Oxford University Press OUP, 1994
ISBN 10: 0199633444
ISBN 13: 9780199633449
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RNA Processing
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RNA Processing: A Practical ApproachVolume I (The ^APractical Approach Series)
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RNA Processing
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RNA PROCESSING, VOL. 1
Published by
SP OXFORD UNIVERSITY PRESS, 1994
ISBN 10: 0199633444
ISBN 13: 9780199633449
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