Synopsis
The detection of specific nucleic acid sequences by in situ hybridization has many important applications in modern biology. In situ detection of viral DNA sequences is enabling the diagnosis of human diseases, the detection of specific sequences on chromosomes is crucial for genome analysis, and in fields such as developmental biology and neurobiology, the investigation of gene expression by in situ hybridization to mRNA has become a key technique. "In Situ Hybridization - A Practical Approach" is a comprehensive practical guide to the uses of this major technique. Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA, and chromosomal DNA. Methods are provided for the analysis of cells, tissue sections, and whole embryos. In addition, techniques are presented for carrying out in situ hybridization in combination with the immunochemical detection of protein, and for using it at the electron microscopic level. This book is aimed at any researcher wishing to exploit the many widesprad applications of this important technique.
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In Situ Hybridization : A Practical Approach
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Oxford University Press, Incorporated, 1992
ISBN 10: 0199633274
ISBN 13: 9780199633272
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In Situ Hybridization (A Practical Approach)
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ISBN 10: 0199633274
ISBN 13: 9780199633272
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Paperback. Condition: Very Good. In Situ Hybridization (A Practical Approach) This book is in very good condition and will be shipped within 24 hours of ordering. The cover may have some limited signs of wear but the pages are clean, intact and the spine remains undamaged. This book has clearly been well maintained and looked after thus far. Money back guarantee if you are not satisfied. See all our books here, order more than 1 book and get discounted shipping. . Seller Inventory # 7719-9780199633272
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In Situ Hybridization: A Practical Approach (The ^APractical Approach Series)
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ISBN 10: 0199633274
ISBN 13: 9780199633272
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In Situ Hybridization (A Practical Approach)
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In Situ Hybridization: A Practical Approach (The ^APractical Approach Series)
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ISBN 10: 0199633274
ISBN 13: 9780199633272
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