Gene Transcription: A Practical Approach: No. 117 - Hardcover

Hames, B. D.

Hardcover

ISBN 10: 0199632928 ISBN 13: 9780199632923

Publisher: Oxford University Press, 1993

Synopsis

Understanding the mechanisms and control of gene transcription is said to be one of the central goals for a large proportion of molecular biologists currently involved in research. This book concentrates on RNA polymerase II transcription of eukaryotic protein-coding systems, but many of the approaches and techniques described apply equally well to the study of systems involving RNA polymerases I and III. This book covers the major current procedures and approaches in this field, including not only the analysis of transcription "in vitro" and "in vivo", but also the identification, purification and characterization of transcription factors and their interaction with specific DNA target sites. It is written by experts and specifically designed for all researchers intent on studying eukaryotic gene expression. Its mix of background information, advice on experimental approaches, detailed practical protocols, typical results, help with troubleshooting and reference appendices make it a guide for the newcomer to the field, and a source of new approaches and techniques for more experienced researchers. David Hames is one of the series editors of the "Practical Approach" series. Other titles in the "PA" series include "Transcription and Translation", "Nucleic Acid Hybridization", "Gel Electrophoresis of Proteins" and "Gel Electrophoresis of Nucleic Acids".

About this title

Review

'The book should be of great value to both the novice and experienced researcher, and presents a broad variety of methods in one convenient volume. The format of step-by-step protocols makes for easy use, introductory and background comments are included, and the tips and trouble-shooting guides should help the worker obtain meaningful results.'Mark Nichols, Gene Expression Programme, EMBI, Heidelberg, Trends in Genetics, January 1994, Volume 10

'gives practical details how to deal with: assay of gene transcription in vitro' International Journal of Biochemistry, Vol. 25, No. 12, 1993

'if you have the 'will' to study transcription factors this book provides the 'way''Dr G. Bilbe, Cell Biology International, Vol. 17, No. 12, 1993

all the protocols are uniform in their format and are well organized ... Each chapter contains useful general introductory material on what can be achieved by the described protocols ... Chapter 1, entitled "Assay of gene transcription in vitro" is especially well done ... All of the chapters contain extensive and timely references. This book is a useful compilation of methodologies for studying mechanisms of transcriptional regulation. (Nisson Schechter, State University of New York, The Quarterly Review of Biology, Volume 69)

About the Author

Stephen J. Higgins, both at the Department of Biochemistry and Molecular Biology, University of Leeds.

Edition details

• Publisher: Oxford University Press
• Publication date: 1993
• ISBN 10: 0199632928
• ISBN 13: 9780199632923
• Binding: Hardcover
• Language: English
• Number of pages: 384