Extracellular Matrix: A Practical Approach (Practical Approach Series): 151 - Softcover

Softcover

ISBN 10: 0199632200 ISBN 13: 9780199632206

Publisher: Oxford University Press, 1993

Synopsis

The extracellular matrix surrounds cells and tissues. It provides structure to organisms and is important in determining cell growth, state of differentiation, and how the cell responds during injury and development. Changes in the extracellular matrix are also important components in both inherited and acquired diseases. Thus studies of the extracellular matrix span much of modern biological research. Because the extracellular matrix resides outside the cell, the properties of the component molecules require special approaches for their study. This volume brings together under a single cover the majority of the basic techniques employed in extracellular matrix research.

About this title

Review

valuable ... It is a multi-authored volume in the superb Practical Approach series and comes packed with protocols on every aspects of ECM research. This is the ECM book that has been needed for a long time, and here are essential protocols for any groups with even the slightest interest in the ECM. I recommend it highly. (Charles Streuli, University of Manchester, Trends in Cell Biology, (Vol.6), November 1996)

This book sets out to provide the research worker with a comprehensive survey and detailed protocols of the biochemical techniques used for studying the macromolecules of the extracellular matrix. The senior authors of all the chapters are well established in the field of their contribution. This is a strong recommendation for a book which should become a standard handbook for anyone working in this field, particularly those just entering or wishing to develop new techniques. (Doreen E. Ashhurst, St George's Hospital Medical School, Cell Biology International, Vol. 20, No. 9, 1996)

From the Back Cover

The importance of the extracellular matrix in maintaining the structural integrity of organisms, in regulating the normal biology of a cell, and in the development and progression of disease, has established this area as a major focus of contemporary research. This book is a timely compendium of the key biochemical, immunological, and molecular biological techniques and approaches currently used to study both extracellular matrix macromolecules and their function in vertebrates. Topics covered include methods for the study of the different genetic types of collagens, the structural glycoproteins (fibronectin, laminin, tenascin, thrombospondin, and SPARC), elastin, glycosaminoglycans, and proteoglycans, for investigating the catabolism of matrix components, for the use of matrix in cell culture, for immunological identification of matrix components in cells and tissues, and for studying the regulation of extracellular matrix gene expression. Extracellular Matrix: A Practical Approach is a unique source of the latest research methods for all researchers in this subject area. It also represents a valuable information resource for investigators whose major interests lie in related fields.

Edition details

• Publisher: Oxford University Press
• Publication date: 1993
• Language: English
• ISBN 10: 0199632200
• ISBN 13: 9780199632206
• Binding: Paperback
• Number of pages: 432
• Editor: HARALSON M.