A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin ¿-related nuclear transport receptors (NTRs)

Language: English

Published by Cuvillier, Cuvillier Aug 2023, 2023

3736978472 / 9783736978478

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This item is printed on demand - Print on Demand Titel. Neuware -Eukaryotic cells are divided into a nuclear and a cytoplasmic compartment. This separates transcription from translation and makes gene expression dependent on nucleocytoplasmic transport. The members of the importin ß superfamily function as shuttling nuclear transport receptors (NTRs) that recognize and actively transport cargoes through nuclear pores. An estimated 5 000 to 10 000 different human proteins are subject to active nuclear transport. Numerous cargo/NTR pairs have been identified, however, we are still far from a complete understanding as it has been very challenging to setup a systematic in vivo analysis that integrates the impact of all transport pathways.In this study, we obtained anti-NTR nanobodies against TRN1, Xpo4, Xpo7, and CAS. Our aim was to identify nanobodies, and prepare nanobody fusions, that impede nuclear pore-passage of the targeted NTR and thus, interrupt a given transport cycle. These nanobody fusions were observed to inhibit the partition of NTR/cargo complexes into a reconstituted FG phase. We also observed that the nanobodies and nanobody fusions inhibit NTR transport in permeabilized cells.Cuvillier Verlag, Nonnenstieg 8, 37075 Göttingen 136 pp. Englisch.…

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Title
A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin ¿-related nuclear transport receptors (NTRs)
Author
Dilantha Perera
Publisher
Cuvillier, Cuvillier Aug 2023
Publication year
2023
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3736978472
ISBN 13
9783736978478
Item weight
187 grams
Dimensions
210x148x8 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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