Sold by Biblioctopus, Los Angeles, CA, U.S.A.
Association Member:
AbeBooks Seller since 20 November 2013
Seller rating 2 out of 5 stars
Sanger received the Nobel Prize in Chemistry in 1958 for determining the complete amino acid sequence of insulin (the first protein ever to have its primary structure fully established) and a second in 1980 for developing methods for sequencing DNA, making him one of only four individuals ever to have received two Nobel Prizes. The two offprints offered here, both signed by Sanger, together constitute the complete published record of that first achievement: a synthetic lecture delivered in Marseille in December 1954 presenting the full sequential strategy by which insulin's structure was approached, and the definitive experimental paper establishing the positions of its three disulphide bridges-the final structural question the sequence work had left open. In demonstrating that a protein possesses a unique, fully definable covalent sequence, Sanger established the conceptual and methodological foundation upon which all subsequent structural biology rests. The principle that biological function is encoded in a specific primary sequence underlies every protein sequenced since, every therapeutic protein produced by recombinant technology, and every drug designed to interact with a defined molecular target; and the sequential degradation strategy Sanger developed for insulin was the direct intellectual precursor of the DNA sequencing methodology for which he received his second Nobel Prize; and which in turn made the Human Genome Project possible. (1) La Structure de l'Insuline; from Bulletin de la Société de Chimie biologique, Tome XXXVII, no. 1, pp.23-35. Paris: Masson et Cie, 1955. Offprint, 8vo (242 x 159mm), pp. 13, [3]. Original blue printed wrappers, staple-bound, some toning and rubbing, else near fine. Signed by Sanger on the front wrapper. A lecture delivered on 13 December 1954 in Marseille, in which Sanger presents a synthetic account of the decade-long experimental program at the Department of Biochemistry, University of Cambridge, that resulted in the complete elucidation of the amino acid sequence of insulin; the first protein ever to have its primary structure fully determined. Setting aside earlier disputed molecular weight values in favor of a minimum covalent unit of 6,000 (later calculated at 5,734), Sanger describes the sequential strategy by which the complete structure was approached: first, the identification of two N-terminal residues (DNP-glycine and DNP-phenylalanine, one equivalent of each) using the fluorodinitrobenzene reagent he had himself introduced in 1945, establishing that insulin comprises two distinct polypeptide chains; then the separation of those chains by performic acid oxidation, which cleaved the cystine disulfide bridges and resolved the molecule into a glycyl chain A of approximately 20 residues and a phenylalanyl chain B of approximately 30; then the stepwise determination of the N-terminal sequences of both chains by partial hydrolysis and partition chromatography, establishing that chain B begins Phe.Val.Asp.Glu and chain A begins Gly.Ileu.Val.Glu.Glu; and finally the mapping of the disulfide bridge positions, including an intrachain ring at positions A6 and A11 whose dimensions Sanger notes are shared by oxytocin and vasopressin, and the two interchain bridges connecting A and B, determined through systematic chymotryptic and peptic hydrolysis under conditions preventing disulfide exchange. The significance of the work extends far beyond insulin: in demonstrating that a protein possesses a unique, fully definable covalent sequence, Sanger established the conceptual and methodological foundation upon which all subsequent structural biology rests, and the principle that biological function is encoded in a specific primary sequence underlies every protein sequenced since, every therapeutic protein produced by recombinant technology, and every drug designed to interact with a defined molecular target. Sanger received the Nobel Prize in Chemistry in 1958 for this work, and a second in 1.
Seller Inventory # 1300
BASICS
Book entries include author (or its equivalent on nonbook items), title (or its equivalent on non¿
book items), place, date, a bibliographical conclusion, and a physical description, typically in that
order. Practical limits in a commercial listing preclude a complete deconstruction, but any aspect
chosen as a digression, for any particular entry, follows the evaluation of many relevant factors at
once, their interdependence, their related importance, and their consequences. We strive to ...
If you are a consumer you can withdraw from the contract in accordance with the following. Consumer means any natural person who is acting for purposes which are outside his trade, business, craft or profession.
Information regarding the right of withdrawal
Statutory right to withdraw
You have the right to withdraw from this contract within 14 days without giving any reason.
The withdrawal period will expire after 14 days from the day on which you acquire, or a third party other than the carrier and indicated by you acquires, physical possession of the last good or the last lot or piece.
To exercise the right of withdrawal, electronically fill in and submit a clear statement on our website, under "My Purchases" in "My Account". We will communicate to you an acknowledgement of receipt of such a withdrawal on a durable medium (e.g. by e-mail) without delay.
To meet the withdrawal deadline, it is sufficient for you to send your communication concerning your exercise of the right of withdrawal before the withdrawal period has expired.
Effects of withdrawal
If you withdraw from this contract, we will reimburse to you all payments received from you, including the costs of delivery (except for the supplementary costs arising if you chose a type of delivery other than the least expensive type of standard delivery offered by us).
We may make a deduction from the reimbursement for loss in value of any goods supplied, if the loss is the result of unnecessary handling by you.
We will make the reimbursement without undue delay, and not later than 14 days after the day on which we are informed about your decision to withdraw from this contract.
We will make the reimbursement using the same means of payment as you used for the initial transaction, unless you have expressly agreed otherwise; in any event, you will not incur any fees as a result of such reimbursement.
We may withhold reimbursement until we have received the goods back, or you have supplied evidence of having sent back the goods, whichever is the earliest.
You shall send back the goods or hand them over to Biblioctopus, Los Angeles, California, U.S.A., +1 3232076708, without undue delay and in any event not later than 14 days from the day on which you communicate your withdrawal from this contract to us. The deadline is met if you send back the goods before the period of 14 days has expired. You will have to bear the direct cost of returning the goods. You are only liable for any diminished value of the goods resulting from the handling other than what is necessary to establish the nature, characteristics and functioning of the goods.
Exceptions to the right of withdrawal
The right of withdrawal does not apply to:
Everything is sent via FedEx, at our expense, but we may take a week to get it wrapped. Purchases under $200, that are to be shipped out of the United States may be sent through a carrier other than FedEx.
| Order quantity | 1 to 12 business days | 1 to 4 business days |
|---|---|---|
| First item | £ 0.00 | £ 0.00 |
Delivery times are set by sellers and vary by carrier and location. Orders passing through Customs may face delays and buyers are responsible for any associated duties or fees. Sellers may contact you regarding additional charges to cover any increased costs to ship your items.
