Microarray Technology: Methods and Applications (Methods in Molecular Biology, 1368)

Hardcover, xiv + 294 pages, NOT ex-library. Gentle handling wear, book is clean with unmarked text, free of inscriptions and stamps, firmly bound. Issued without a dust jacket. -- A comprehensive guide to the rapidly evolving field of microarray technology, detailing both its methodology and its wide-ranging applications in biological and medical research. It offers in-depth coverage of how microarrays are used for high-throughput analysis of nucleic acids, proteins, peptides, glycans, and even whole cells, demonstrating their pivotal role in genomics, proteomics, and other omics sciences. The text examines cutting-edge developments, including new probe immobilisation techniques, microRNA profiling, and the use of microarrays in diagnostic and therapeutic contexts. It provides detailed protocols for the design, development, and application of microarrays in various assays, as well as insights into bioinformatics and data analysis tools that complement microarray experiments. The book serves as an invaluable resource for researchers in biotechnology, molecular biology, and medical diagnostics, offering practical advice on improving assay sensitivity and specificity. Key advancements such as microfluidics integration and next-generation sequencing are also explored, highlighting how these innovations enhance the precision and capabilities of microarray platforms in detecting complex biomolecular interactions and disease biomarkers. -- Contents: Part I Introduction -- 1 Overview of Microarray Technology / Paul C.H. Li; Part II Advances on Techniques in Microarray Technology -- 2 Surface Functionalization for Immobilization of Probes on Microarrays / C. Desmet & C.A. Marquette; 3 The 'Clickable' Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays / Tomasz Ratajczak; 4 Microarray Developed on Plastic Substrates / María-José Banuls; 5 Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray / Ryo Iizuka, Taro Ueno & Takashi Funatsu; 6 Probe Design Strategies for Oligonucleotide Microarrays / Nicolas Parisot; Part III Applications of Microarray Technology to Nucleic Acid Assays -- 7 Analyzing Illumina Gene Expression Microarray Data Obtained from Human Whole Blood Cell and Blood Monocyte Samples / Alexander Teumer; 8 Microarray Technology and Its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers / Hirotaka Konishi; 9 Application of DNA Microarray to Clinical Diagnostics / Ankita Patel & Sau W. Cheung; 10 High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device / Abootaleb Sedighi & Paul C.H. Li; 11 Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies / Annalisa Lonetti; 12 DNA Microarray-Based Diagnostics / Mahsa Gharibi Marzancola; Part IV Applications of Microarray Technology to Other Biological Assays -- 13 High-Throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays / Liqian Gao; 14 Nanotechnology in the Fabrication of Protein Microarrays / Manuel Fuentes; 15 Epitope Mapping Using Peptide Microarray in Autoantibody Profiling / Sebastian Henkel; 16 Preparation of Glycan Arrays Using Pyridylaminated Glycans / Shin-ichi Nakakita & Jun Hirabayashi; 17 Competitive Immunoassays Using Antigen Microarrays / Zhaowei Zhang; 18 Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays) / Frédérique Deiss, Yang Yang & Ratmir Derda; 19 Cell Microarrays for Biomedical Applications / Mario Rothbauer, Verena Charwat & Peter Ertl; Index.