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Wiesbaden, Verlag von J.F. Bergmann, 1905, Gr.-8°, VIII, 162 pp., 13 Abbildungen, 7 Bl. Verlagsanzeigen, orig. Broschur. First Edition! Ivar Christian Bang "Founder of Modern Clinical Microchemistry" ! If anyone, be called the founder of modern clinical chemistry . he had gone so far in laying the foundation of concept and practice in modern clinical chemistry, that he may well be called its founder" Van Slyke observed that Bang introduced at least three new techniques to biochemistry: (a) the use of micro- samples of blood weighed with a torsion balance, and absorption of the blood on prepared filter paper, (6) steam distillation of ammonia in Kjeldahl microanalysis; and (c) centrifugation to separate and wash quantitative precipitates. cf. - Vagn Schmidt, Ivar Christian Bang (1891-1918), Founder of Modern Clinical Microchemistry, Clinical Chemistry, 32/1896), pp.213-215 "In Europe, apart from Otto FOLIN's influence, the development by Ivar Christian BANG (1869-1918) of the "micro-analysis of blood constituents" was particularly important for analytical methodology in clinical chemistry. For clinical purposes, there were hardly any methods available before the First World War for examining blood constituents. To measure glucose in blood at least 20 to 30 ml of blood were necessary. It was enormously complicated so that in medical practice nobody tried to measure glucose. Since 1906 Ivar BANG had been working on improving "blood sugar methods". In 1913 he published (Bang, I., Larson, K.A., Über die Bestimmung einiger Blutbestandteile. Biochem. Z. 51, pp.193-199) a completely new "method for microanalysis of blood constituents". This method permitted the quantitative analysis of - chloride, glucose, dry weight, total-N, non-protein nitrogen, hemoglobin, albumin and globulin (Methoden zur Mirkobestimmung einiger Blutbestanteile (1913), p.3). Each test used just 100 to 150 microlitres of blood, a drop from the finger pad. The blood is "soaked up using a small piece of filter paper. By appropriate treatment with a solution the extractable substances are removed, the protein remaining in the paper. Finally using suitable micro-analytical methods the dissolved constituents can be quantified. The filter paper is weighed before and after soaking with blood, the difference comprising the applied amount of blood". BANG used a rapid torsion balance for weighing, and titration for the analytical measurements. BANG also simplified PREGL's micro-Kjeldahl determination (determination of nitrogen by sulphuric acid digestion). In just 18 years in Lund, BANG published a great number of articles on experiments in completely different areas of physiological chemistry, which for reasons of space can not be described here. We should, however, mention that it was his suggestion, to use a centrifuge in microchemistry for the separation of precipitations." Johannes Büttner, Roots of Clinical Chemistry, pp.104-105.
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