Published by Woodhead Publishing Ltd 2014-10-01, 2014
ISBN 10: 1907568921 ISBN 13: 9781907568923
Language: English
Seller: Chiron Media, Wallingford, United Kingdom
Hardcover. Condition: New.
Seller: Revaluation Books, Exeter, United Kingdom
Hardcover. Condition: Brand New. 1st edition. 174 pages. 9.50x6.25x0.75 inches. In Stock.
hardcover. Condition: Sehr gut. 188 Seiten; 9781907568923.2 Gewicht in Gramm: 500.
Published by Woodhead Publishing Ltd, 2014
ISBN 10: 1907568921 ISBN 13: 9781907568923
Language: English
Seller: Brook Bookstore On Demand, Napoli, NA, Italy
Condition: new. Questo è un articolo print on demand.
Buch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.