Springer Us Jan 2021 (28 results)
Language: English
Published by Springer US Jan 2021 2021
Series: Springer Protocols Handbooks, Book 42 of 84. Book 42 of 84 - Springer Protocols Handbooks
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based o…n a thorough review and analysis of the current literature as well as the authors' experiences in the lab.Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy. 136 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 740 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimension…al gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field. 240 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition volume delivers updated and new chapters on xenotransplantation covering a variety of methods.Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in th…e last three decades. Expert researchers provide characterizations of xenogeneic interactions at the cellular and molecular levels, detailed descriptions of complex transplantation models, as well as attractive new tools for further advancement including 3D cell-culturing systems and genomic editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and intuitive,Xenotransplantation: Methods and Protocol, Second Editionaids scientists in continuing to study xenotransplantation and its multiple aspects. 376 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details state-of-art eQTL analysis, where interdisciplinary researchers are provided both theoretical and practical guidance to eQTL analysis and interpretation. Chapters guide readers through methods and tools for eQTL…and QTL analysis and the usage of such analysis in various scenarios.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, eQTL Analysis: Methods and Protocols to ensure successful results in the further study of this vital field. 264 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion c…ell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems. 248 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing c…ritical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. 416 pp. Englisch.
Language: English
Published by Springer US Jan 2021 2021
Series: Springer Protocols Handbooks, Book 43 of 84. Book 43 of 84 - Springer Protocols Handbooks
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosop…hila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field. 260 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 312 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological act…ivity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research. 388 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation… assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field. 164 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, pr…otocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks. 320 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study th…e T cell activation, techniques to study subsets of Tcell's, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers. 308 pp. Englisch.
Language: English
Published by Springer US, Springer New York Jan 2021 2021
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell…morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 248 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell m…igration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs. 252 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such…as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines. 592 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume looks at the latest techniques used by researchers to help them understand the biology of various cellular processes and agronomic traits, and come up with better strategies to improve legume crops. The chapters in this…book cover topics such as legume genomic resources; legume pangenome and organelle genome construction; transcriptome analysis; DNA methylation analysis; double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture; genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures; genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and informative, Legume Genomics: Methods and Protocols is a useful reference for genomicists, molecular breeders, plant molecular biologists, biotechnologists, computational biologists, and developmental biologists. This book is also an excellent resource for any novice and expert researcher involved in various molecular aspects of legume biology or general plant studies. 424 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative mo…dels to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics. 280 pp. Englisch.
Language: English
Published by Springer US, Chapman And Hall/CRC Jan 2021 2021
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides a comprehensive guide to scientists, engineers, and students that employ metabolomics in their work, with an emphasis on the understanding and interpretation of the data. Chapters guide readers through common tool…s for data processing, using database resources, major techniques in data analysis, and integration with other data types and specific scientific domains.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, practical guidance of methods and techniques, useful web supplements, and connect the steps from experimental metabolomics to scientific discoveries.Authoritative and cutting-edge,Computational Methods and Data Analysis for Metabolomicsto ensure successful results in the further study of this vital field. 504 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware 312 pp. Englisch.
Language: English
Published by Springer US Jan 2021 2021
Series: Methods in Molecular Biology, Book 90 of 382. Book 90 of 382 - Methods in Molecular Biology
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient met…hods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties. 396 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition provides new and updated chapters useful for the study of Regulatory B cells. Organized in four sections, chapters detail basic methods for the isolation and immunophenotypical analysis of these cells, experimental appr…oaches for the ex vivo generation/expansion of IL-10 producing B cells, molecular biology techniques for the analysis of IL-10 expression and production, and animal models mimicking pathologic settings. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Regulatory B Cells: Methods and Protocols, Second Edition aims to be useful to the scientific community and serve to clarify some unsolved aspects of Regulatory B Cells research. 488 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters…include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field. 256 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, settin…g up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field. 364 pp. Englisch.
Language: English
Published by Springer US, Springer US Jan 2021 2021
Series: Methods in Molecular Biology, Book 90 of 382. Book 90 of 382 - Methods in Molecular Biology
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Buch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods… for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 396 pp. Englisch.
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and explor…ing ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field. 516 pp. Englisch.
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational… modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well. 608 pp. Englisch.
Language: English
Published by Springer US, Springer New York Jan 2021 2021
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Buch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational mod…ifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 608 pp. Englisch.
