springer new york springer us sep 2018 (23 results)

Buch. Condition: Neu. Neuware -Fifty years ago, a new approach to reaction kinetics began to emerge: one based on mathematical models of reaction kinetics, or formal reaction kinetics. Since then, there has been a rapid and accelerated development in both deterministic and stochastic kinetics, primarily because mathematicians studying differential equations and algebraic geometry have taken an interest in the nonlinear differential equations of kinetics, which are relatively simple, yet capable of depicting complex behavior such as oscillation, chaos, and pattern formation.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 496 pp. Englisch.

Buch. Condition: Neu. Neuware -This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 216 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 476 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.Cutting-edge and comprehensive, Pre-Eclampsia: Methods andProtocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 372 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Innate Immune Activation: Methods and Protocolsaims to be a useful and informative reference tool for further study into this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 396 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 468 pp. Englisch.

Buch. Condition: Neu. Neuware -This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Buch. Condition: Neu. Neuware -This book describes approaches for the analysis of plant pathogenic fungi that range from ecosystem composition to in vitro and wet-lab to computational analyses. Chapters detail bioinformatics protocols for genome assembly, transcriptome analysis and small RNA profiling, characterization of secondary metabolome, analysis of post-translational modification and localization of proteins, examination of virulence function and interaction with plants or other microorganisms, and targeted mutagenesis using various approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through awide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 304 pp. Englisch.

Buch. Condition: Neu. Neuware -This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 360 pp. Englisch.

Buch. Condition: Neu. Neuware -This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 296 pp. Englisch.

Buch. Condition: Neu. Neuware -The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls.Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 304 pp. Englisch.

Buch. Condition: Neu. Neuware -This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 240 pp. Englisch.

Buch. Condition: Neu. Neuware -This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 292 pp. Englisch.

Buch. Condition: Neu. Neuware -This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 324 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -Looking to the future, the editors predict that the foundational information provided in this book -- combined with the advent of new tools and budding interests in use of non-rodent mammalian models -- will produce another major advance in knowledge regarding the biology of spermatogonia over the next decade. In particular, we anticipate that the core molecular machinery driving different spermatogonial states in most, if not all, mammals will be described fully, the extrinsic signals emanating from somatic support cell populations to influence spermatogonial functions will become fully known, and the capacity to derive long-term cultures of SSCs and transplant the population to regenerate spermatogenesis and fertility will become a reality for higher order mammals.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 400 pp. Englisch.

Buch. Condition: Neu. Neuware -This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Cancer Epigenetics for Precision Medicine: Methods and Protocols is a valuable resource to help researchers and scientists identify these specific biomarkers and work towards the prevention, diagnosis, and prognosis of different cancers in the future.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 312 pp. Englisch.

Buch. Condition: Neu. Neuware -Atlas of Lung Pathology offers a comprehensive survey of common and uncommon conditions likely to be encountered in a wide range of practices. It begins with a review of normal macroscopic and microscopic lung anatomy followed by practical tips on how to handle the most common specimens received in pathology practice. Non-neoplastic conditions are segregated into logical categories intended to make them easily accessible to quickly offer solutions for diagnostic problems encountered in daily practice. Each illustration was carefully chosen using images drawn from experienced contributors familiar with the challenges of diagnostic pulmonary pathology.¿Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.

Buch. Condition: Neu. Neuware -¿This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.¿Thorough and authoritative, The UbiquitinProteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 432 pp. Englisch.

Taschenbuch. Condition: Neu. Neuware -This handbook collates research evidence and presents the most up-to-date findings on child development in Sub-Saharan Africa. It discusses complex risk factors and medical conditions affecting childhood outcomes, and spotlights emerging programs for enhancing literacy and cognitive development. The panel of expert contributors offer needed context and knowledge to the discussion of previously understudied topics. Chapters present proven intervention strategies currently in use across the diverse region. In addition, this handbook provides guidelines for culturally sensitive and ethical research that will inform practice and help shape policy goals and initiatives. Topics featured in the Handbook include: Fatherhood in the African context. Sibling care-giving and its implications in Sub-Saharan Africa. Nutritional status, infections, and child development Diabetes in Sub-Saharan African children. How to adapt tests for Sub-Saharan Africa. Interventions aimed at children and caregivers. A culturally sensitive approach to conducting research and promoting initial literacy development in AfricaThe Handbook of Applied Developmental Science in Sub-Saharan Africa is a must-have resource for researchers, professionals/scientist-practitioners, and graduate students in child, school, and developmental psychology, as well as pediatrics, social work, public health, and education.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 436 pp. Englisch.

Buch. Condition: Neu. Neuware -This book presents a comprehensive collection of detailed state-of-the-art exon skipping and splices modulation protocols. Chapters detail 14 genetic diseases, AON-mediated therapies, and CRISPR/Cas9-mediated gene editing therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Exon Skipping and Inclusion Therapies: Methods and Protocols aims to help researchers initiate the development of next-generation therapies.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 588 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book offers physiology teachers a new approach to teaching their subject that will lead to increased student understanding and retention of the most important ideas. By integrating the core concepts of physiology into individual courses and across the entire curriculum, it provides students with tools that will help them learn more easily and fully understand the physiology content they are asked to learn. The authors present examples of how the core concepts can be used to teach individual topics, design learning resources, assess student understanding, and structure a physiology curriculum.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 172 pp. Englisch.

Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Since publication of the first edition in 1994, the second edition in 1999, and the third edition in 2009, many new advances in sleep medicine have been made and warrant a fourth edition. This comprehensive text features 19 additional chapters and covers basic science, technical and laboratory aspects and clinical and therapeutic advances in sleep medicine for beginners and seasoned practitioners.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 1320 pp. Englisch.