humana press inc totowa nj (97 results)

Hardcover. Condition: Very Good. Dust Jacket Condition: No Dust Jacket. First Edition. Govt.Ref.Lib.Copy. Hardly Used. Library sticker on spine and FEP; library stamp on FEP; text tight and bright. Used.

Paperback. Condition: Good. David Kuter and a host of leading international researchers summarize in one volume all the knowledge of thrombopoietins (TPO) available today. The distinguished experts review the history of the search to discover TPO, describe the molecular and biological characteristics of this new molecule, and present the results of the preclinical animal experiments that will guide clinical use of this new hormone. Along the way they provide the most recent and comprehensive guide to the biology of megakaryocytes and platelets. The book has been read but remains in clean condition. All pages are intact and the cover is intact. Some minor wear to the spine.

Hardcover. Condition: As New. No Jacket. Glossy illustrated boards. 1st printing, 2006. No jacket as published. Like New. Firmly bound, unread. Crisp, bright and unmarked. No remainder mark. Like New condition.

Hardcover. Condition: New. No Jacket. Humana Press Inc.,U.S. hardback, 2004, A BRAND NEW BOOK IN PRISTINE ORDER, UNUSED. Full refund if not satisfied. 24 hour despatch.

Paperback. Condition: new. Paperback. Imaging of the airways is a fascinating undertaking and, if done expertly, a true art in medicine. Over the past years, imaging of the airways has become ever more exciting and demanding, as new therapies in pulmonary medicine have become available that require careful imaging assessment of patients to aid management and monitor therapeutic success. The increasing importance of imaging in pulmonary medicine is accompanied by a surge of revolutionary developments in imaging technology that enable ever finer assessment of lung structure and function. No modality has seen more rapid innovation over the last decade than computed tomography. Current multidetector-row CT technology makes high-resolution imaging of the chest with a spatial resolution of a couple of 100 m in a matter of mere seconds. Ever more sophisticated post-processing applications enable intuitive, quantitative assessment of lung structure and function in an efficient manner, so that these technologies are increasingly embraced in mainstream medicine to improve patient care. CT of the Airways, which was so expertly assembled by my dear friends Phil Boiselle and David Lynch, is one of the very first tomes that pays tribute to our new found prowess in airway imaging. Over the past years, imaging of the airways has become ever more exciting and demanding, as new therapies in pulmonary medicine have become available that require careful imaging assessment of patients to aid management and monitor therapeutic success. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Paperback. Condition: new. Paperback. A comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of sexually transmitted diseases, including HIV. The methods-each crafted by a leading expert for comprehensiveness and genuine laboratory utility-cover the major sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes simplex. Additional molecular techniques are included for detecting the genital mycoplasmas, hepatitis B virus, HIV-1, human papillomavirus. Sexually Transmitted Disease Protocols provides a comprehensive collection of foolproof molecular methods for STD diagnosis, research, and patient management. In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- standing of sexually transmitted diseases. Shipping may be from our UK warehouse or from our Australian or US warehouses, depending on stock availability.

Hardcover. Condition: new. Hardcover. This third edition of Gershwin and Halpern's classic text, Bronchial Asthma, has been completely rewritten and expanded to include, from a variety of disciplines, the latest techniques and developments in the clinical diagnosis and treatment of asthma. With its new patient-oriented chapters on living with asthma, the book will empower the internist, the family practitioner, and the specialist in their struggle to reverse the current trend of increasing asthma mortality and to improve the quality of life of their patients and their families. The editors have selected authoritative contributors to Bronchial Asthma, organizing it into four comprehensive parts: I. Definitions and Host Responses to Bronchospasm defines asthma by analyzing its epidemiology, pathogenesis, and genetic roots, and illuminates the roles of mast cells and eosinophils. Chapters also cover the use of the pulmonary function laboratory and the differential diagnosis of asthma in children and adults. II.Patient Management introduces the latest thinking and techniques about treatment and management of patients, including allergic evaluation, radiological treatment, the role of immunotherapy, the differential management of status asthmaticus, the management of pregnant asthmatics, and an evaluation of unconventional therapies. III. Special Clinical Problems explores respiratory tract infections, the roles of anti-inflammatory agents, food additives, air pollution, aspergillosis, and occupational asthma. Four new chapters analyze exercise-induced asthma, asthma's relationship with chronic sinus disease, and the cross-cultural treatment of asthma in Europe and Japan. IV. Living with Asthma, an entirely new section, examines asthma in school children, athletes and asthma, legal issues for asthmatics, the psychology of asthma, and the use of prospective disease management. This textbook is divided into four parts: definitions and host responses to bronchospasm; patient management; special clinical problems; and living with asthma. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability.

Hardcover. Condition: new. Hardcover. DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology. Initially an application for mRNA, DNA microarray technology is now used for many applications, some from large commercial providers. This book explores those applications and includes details to design and produce one's own probes and arrays. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney. This cutting-edge book delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney. It includes step-by-step instructions providing a level of detail rarely found in journal articles. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs. In this book, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field. This book examines various techniques currently used in the rapidly-developing field of neural transplantation for CNS repair. From the laboratory to the clinic it provides thorough and essential protocols to aid current and future neuroscientists. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics. Yeast-based functional genomics and proteomic technologies have increased our understanding of bacterial, yeast, fly, worm and human gene functions. In this book, experts in the field offer stand-alone protocols suitable for daily use in research laboratories. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Epilepsy is fairly unique among the various neurological disorders as it provides the neuroscientist with almost boundless opportunities to examine basic neurobiological mechanisms. Not surprisingly, advances in epilepsy research are closely tied to dev- opment of innovative neurobiological methodologies. In many cases the practical application of these innovations - especially in the context of a neurological disorder with anatomical, molecular, electrophysiological, and behavioral components such as epilepsy - can be found in the development of new animal models. In turn, our understanding of the pathogenesis of epilepsy (and new therapy development) greatly benefits from these models. Taking advantage of transgenic and homologous rec- binationtechniques,laboratorieshave recentlymovedbeyondthestandardconvulsant or stimulation models in rat to develop novel mouse models of epilepsy. This forward thinking approach has recently been applied to genetically tractable "simple" species such as Drosophila melanogaster (fruit flies), Caenorhabditis elegans (worms), Xenopus laevis (tadpoles), and Danio rerio (zebrafish).With contributions from prominent investigators in this field, this book provides a reviewoftheseemerginganimalmodelsofepilepsy. Priortextbooksdevotedtomodels of seizure and epilepsy almost exclusively categorized rat models with little attention paid to these more innovative approaches. Here we attempt to diverge from the conventionalepilepsyliteratureandfocusonanimalmodelsthatattempttoincorporate the latest technological advancements in neurobiology. While some of these models andapproaches are,admittedly, at veryearly stages ofdevelopment andmay ultimately fall short of widespread utilization, it is through the consideration and presentation of these models that the authors' hope to advance and challenge the field of epilepsy research. In this book, investigators examine several novel models of epilepsy in mice as well as in genetically tractable "simple" species. This is an ideal guide for scientists who wish to expand our understanding of the pathogenesis of this neurological disorder. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques, common bioinformatics and annotation tools to glean relevant driver genomic events, and different performance characteristics as well as quality metrics necessary for the robust validation of tumor profiling as a diagnostic test for medical laboratories. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols aims to be a useful resource for learning about technical details, applications, and strengths and limitations of the latest technologies as applied to this increasingly important field. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come. Bacterial Persistence Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc. ). The principal function of meiosis is to reduce the genome complement by half, which is accomplished through sequential execution of one round of DNA replication followed by two rounds of chromosome segregation. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Recombination also serves to increase genetic diversity from one generation to the next by breaking up linkage groups. The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identification of meiosis-defective mutants and cloning of the genes involved; (2) development of direct physical assays for DNA intermediates and products of recombination; and (3) increasingly sophisticated cy- logical methods that describe chromosome behaviors and the spatial and temporal patterns by which specific proteins associate with meiotic chromosomes. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade. Although well appreciated, the structural and biochemical complexity and the dynamic nature of the living matrix are still under extensive inv- tigation, yielding a growing number of methods with varying degree of sophistication and intricacy. In this edition of Extracellular Matrix Protocols, we compiled a variety of methods that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are indeed available in a limited number of centers, but are well worth the awareness and exposure to the ECM research community. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. All chapters were contributed by scientists who developed the methods or mastered and perfected methods that were routinely used in their laboratories. An effort was made to provide practical working details and helpful notes for the nonexpert user in order to assist reproducibility and accuracy. We hope that these valuable protocols will become helpful tools for ECM researchers and will be further developed and tailored to the s- cific needs of a growing number of applications. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. The transfer of hereditary information from genes to proteins is one of the essential pr- esses in all living organisms on our planet. Some genes are expressed without modu- tion throughout the life of a cell, while many others require various degrees of control to precisely balance cellular metabolism with environmental conditions. For many years, researchers attributed this regulatory function to protein molecules, which can direct gene expression at multiple levels, in response to various input signals, and with different degrees of selectivity. Even when the control of gene expression was achieved via direct interactions between proteins and mRNAs, the active role was routinely assigned to p- teins, while RNAs were considered merely as recipient molecules. The discovery of RNA interference and multiple bacterial regulatory RNAs caused a shift from the perception of proteins as the predominant regulators of gene expression to the acknowledgement of the importance of RNAs in many regulatory circuits. Such a viewpoint received strong support several years ago after the discovery of riboswitches and related RNA sensors mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. These classes of RNA pass on cellular and environmental information directly to transcription or translation machinery without the assistance of proteins. The riboswitches are commonly defined as evolutionarily conserved mRNA regions capable of specific binding to metabolite molecules, and, as a result, adopting a particular RNA conformation that modulates gene expression. The discovery of RNA interference and multiple bacterial regulatory RNAs caused a shift from the perception of proteins as the predominant regulators of gene expression to the acknowledgement of the importance of RNAs in many regulatory circuits. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Acute Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterization of genome-wide epigenetic changes in leukemic cells.Written in the highly successful Methods in Molecular Biology series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Acute Leukemia: Methods and Protocols will help researchers to advance knowledge and have a better understanding of the disease, which will ultimately facilitate development of anti-cancer therapy and improve quality of life for patients. Part of the highly successful Methods in Molecular Biology(TM) series, this collection by leading researchers brings together a range of state-of-the-art laboratory methods and detailed protocols that are useful both for all scientists working on the disease. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Biomarkers are molecular indicators of a biological status and, as biochemical species, can be interrogated to evaluate disease status and therapeutic interventions. Biomarkers may be detectable in the blood, other body fluids, or tissues. The expectation is that the level of an informative biomarker is related to the specific type of disease present in the body. Hence, disease-relevant biomarkers can be used to measure the presence, progress, or intensity of disease. Through a variety of mechanisms, cancer cells provide the biomarker material for their own detection. Tumor biomarkers include cancer-specific mutations or changes in gene expression, both of which can result in aberrant protein expression. These variant or abundant proteins can be detectable in the circulation as the free proteins or as novel autoantibodies to those proteins, the latter indicating that the immune system can provide an exquisitely sensitive sensor of disease. Because cancer cells shed DNA in the circu- tion, an event rarely seen in healthy individuals, tumor-specific genetic changes, such as promoter methylation or gene mutations, are detectable in DNA prepared from plasma or other body fluids. Cancer-related biochemical changes often effect measurable me- bolic variations within a cell or organism. In addition, these biochemical changes result in posttranslational modification of proteins via glycosylation or phosphorylation providing a plethora of opportunity for biomarker discovery. These variant or abundant proteins can be detectable in the circulation as the free proteins or as novel autoantibodies to those proteins, the latter indicating that the immune system can provide an exquisitely sensitive sensor of disease. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. The G protein-coupled receptors (GPCRs) and associated peripheral G proteins underpin a multitude of physiological processes. The GPCRs represent one of the largest superfamilies in the human genome and are a significant target for bioactive and drug discovery programs. It is estimated that greater than 50% of all drugs, including those in development, currently target GPCRs. Many of the characterized GPCRs have known ligands; however, approximately 20% of GPCRs are described as orphan GPCRs, apparent GPCRs that share the generic high-level structure charact- istic of GPCRs but whose endogenous ligand is not known. Therefore, it is expected that the field of GPCR drug discovery and development will greatly expand in the coming years with emphasis on new generations of drugs against GPCRs with unique therapeuticuseswhichmayincludedrugssuchasallostericregulators,inverseagonists, and identification of orphan GPCR ligands. AswelearnmoreaboutthemolecularsignalingcascadesfollowingGPCRactivation, we acquire a better appreciation of the complexity of cell signaling and as a result, also acquire a vast array ofnew molecularmethods toinvestigate these andother processes. Thegeneralaimofthisbookistoprovideresearcherswitharangeofprotocolstha tmay be useful in their GPCR drug discovery programs. It is also the basis for the devel- ment of future assays in this field. Therefore, the range of topics covered and the appropriate methodological approaches in GPCR drug discovery are reflected in this book. Itisinterestingtonotethatfuturedirectionsindrugdiscoverywillrequireinput and collaboration from a plethora of fields of research. As such, this book will likely be of interest to scientists involved in such fields as molecular biology, pharmacology, biochemistry, cellular signaling, andbio-nanotechnology. Therefore, it is expected that the field of GPCR drug discovery and development will greatly expand in the coming years with emphasis on new generations of drugs against GPCRs with unique therapeuticuseswhichmayincludedrugssuchasallostericregulators,inverseagonists, and identification of orphan GPCR ligands. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system. In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today. Controversy surrounds the study of human embryonic stem cells. In this book, respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping. Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies. This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. In a state-of-the-art synthesis of basic science and clinical practice, Roy Smith and a distinguished panel of researchers and clinicians review GH regulation and its action at the molecular level, and describe the basis for GH deficiency and the use of GH as therapy in a variety of clinical situations. The clinical presentation moves beyond the treatment of GH-deficient children to include the genetics of GH-deficiency, GH-deficiency in adults, osteoporosis, Syndrome X, sleep quality, GH in AIDS patients, GHRH in clinical studies. Timely and innovative, Human Growth Hormone: Research and Clinical Practice will benefit both basic and clinical researchers, as well as those clinical endocrinologists who want to use growth hormone not only in treating children, but also in treating adult disorders, including those associated with metabolic disease. In a state-of-the-art synthesis of basic science and clinical practice, Roy Smith and a distinguished panel of researchers and clinicians review GH regulation and its action at the molecular level, and describe the basis for GH deficiency and the use of GH as therapy in a variety of clinical situations. Shipping may be from our UK warehouse or from our Australian or US warehouses, depending on stock availability.

Hardcover. Condition: new. Hardcover. This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance. Chapters cover topics ranging from new molecular and cellular mechanisms of tolerance; generation and characterization of mice regulatory macrophages; recent advances in the treatment of immune-mediated inflammatory disorders; and novel mechanisms and therapeutic perspectives on food allergies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II is a great resource for current research and inspiration for new studies in immune tolerance. Suppression and Regulation of Immune Responses Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations. This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.

Hardcover. Condition: new. Hardcover. This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results. This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.