Zymography : Biological and Clinical Applications, Volume 2

Language: English

Published by Springer, 2026

107164484X / 9781071644843

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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Druck auf Anfrage Neuware - Printed after ordering - Volume 2 describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 2 are organized in three parts. Part I introducesin situzymography and localization of bright green-fluorescent gelatinase activity in tissue sections,in situzymography in formalin-fixed paraffin-embedded and mineralized tissues, andin vivozymography as an essential activity assay for studying the activity of matrix metalloproteinases (MMPs) in a cell-specific manner in the brain. Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates. Part III focuses on potential clinical applications of zymography, with chapters describing assessment of MMP-2 and MMP-9 hydrolytic activity in preclinical and clinical tissue samples, the use of zymography to assess circulating MMP-2 and MMP-9 in plasma and serum and in pathological conditions, and the use of zymography for the detection of bacterial proteases. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Zymography: Biological and Clinical Applications, Volume IIis a valuable resource for both experts in the field, as well as new scientists aspiring to learn and perform successful zymography techniques.

Seller Inventory # 9781071644843

Title
Zymography : Biological and Clinical Applications, Volume 2
Author
Raouf A. Khalil
Publisher
Springer
Publication year
2026
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
107164484X
ISBN 13
9781071644843
Item weight
561 grams
Dimensions
254x178x17 mm

AHA-BUCH GmbH

Einbeck, Germany

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