Variability Anlysis In Immunogenic Region Of Hepatitis B Virus: Sequence Variation In Pakistanian Hepatitis B Virus

Abbas, Naaz; Abbas, Naaz

ISBN 10: 3639149084 / ISBN 13: 9783639149081

Published by Vdm Verlag Dr. Müller, 2009

152 pages. 8.66x5.91x0.35 inches. In Stock. Seller Inventory # 3639149084

Synopsis:

The work was carried out to analyze variability in the immunogenic regions of surface envelope genes of Pakistanian Hepatitis B virus isolates. The PreS region, the hypervariable and highly immunogenic region, containing at least five epitopes, was PCR amplified, cloned and sequenced. Sequence alighnments and comparisons with other known HBV sequences from different parts of the world were performed in order to build a phylogenetic tree and to check if certain amino acids mutations tend to recur in certain places within the hypervariable region. On the basis of Phylogenetic analysis of PreS1 region from 12 newly sequenced Pakistanian HBV isolates, it appears that Genotype C is the most prevalent genotype in Pakistan and then comes Genotype D. It was also concluded that four mutations shared by NA004, NA018 and NA020 may be prevalent mutations in the Pakistanian ayw subtype. Six novel sequences and 13 mutations explored during this study may be specific to Pakistanian HBV isolates hence this information may be potentialy valuable for diagnostic puposes and for HBV vaccine development in Pakistan.

About the Author: Msc Zoology, Ph D molecular Biology,Post doctrate SBS, University of the punjab, Lahore Pakistan. Visiting Professor University of the punjab. HEC approved Ph D supervisor. Principal scientific officer, FBRC, PCSIR Laboratories, Lahore Pakistan.

Bibliographic Details

Title: Variability Anlysis In Immunogenic Region Of...
Publisher: Vdm Verlag Dr. Müller
Publication Date: 2009
Binding: Paperback
Condition: Brand New