Two Hybrid Technologies. Methods and Protocols
Language: English
Published by Humana Press, New York, 2012
- Hardcover
- Used
Seller: Research Ink, Takoma Park, MD, U.S.A.Research Ink
AbeBooks seller since August 1, 2000
Condition: Used - As new
£ 49.21
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Add to basketItem description from seller
xi + 329 pp. Methods in Molecular Biology, volume 812.
Seller Inventory # 27157
- Title
- Two Hybrid Technologies. Methods and Protocols
- Author
- Suter, Bernhard, and Erich E. Wanker, Eds.
- Publisher
- Humana Press, New York
- Publication year
- 2012
- Condition
- As new
- Book Type
- book
- Binding
- Hardback
- Language
- English
- ISBN 10
- 1617794546
- ISBN 13
- 9781617794544
- Seller catalogs
- Physiology
Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.
"Synopsis" may belong to another edition of this title.
From the Back Cover
Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.
"About the title" may belong to another edition of this title.
Research Ink
Takoma Park, MD, U.S.A.
AbeBooks seller since August 1, 2000
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