Studies on Polygalacturonase Production from Isolated BacillusC4

Language: English

Published by LAP LAMBERT Academic Publishing Jul 2015, 2015

3659752657 / 9783659752650

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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermanyBuchWeltWeit Ludwig Meier e.K.

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Softcover

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This item is printed on demand - it takes 3-4 days longer - Neuware -The present investigation is envisaged to study the production of alkaline and thermostable polygalacturonase using isolated soil Bacillus subtilis(C4) by submerged fermentation. A total of 66 bacterial colonies were isolated from six sampling sites. Out of 66 isolates 8 isolates showed polygalacturonase activity and C4 isolate showed maximum activity. The new isolate polygalacturonase potential was tested against three polygalacturonase producing microorganism. Comparatively isolate C4 showed maximum enzyme activity hence it was chosen for in depth study. The isolate C4 was characterized and identified as Bacillus subtilis C4. The new Bacillus subtilis was used for polygalacturonase production under submerged ferementation Growth profile of the above isolate was studied. The results revealed that the organism was showing maximum log phase till 24hr period after which the growth gradually reduced and remained stable till 72h indicating stationary phase at 400C and pH 11.0. Process parameters like incubation time (19.65U/mL), temperature (28.08 U/mL),pH 7.0, inoculum volume(1mL), inoculum age (24h) and agitation (150rpm) were studied for maximum production of polygacturonase. 156 pp. Englisch.…

Seller Inventory # 9783659752650

Title
Studies on Polygalacturonase Production from Isolated BacillusC4
Author
M. P. Kusuma
Publisher
LAP LAMBERT Academic Publishing Jul 2015
Publication year
2015
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3659752657
ISBN 13
9783659752650
Item weight
250 grams
Dimensions
220x150x10 mm

BuchWeltWeit Ludwig Meier e.K.

Bergisch Gladbach, Germany

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