Simple DNA Extraction Methods from Human Blood : A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and Chelex-100 for DNA Extraction from Human Blood Samples

Language: English

Published by LAP LAMBERT Academic Publishing, 2014

3659536342 / 9783659536342

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nach der Bestellung gedruckt Neuware - Printed after ordering - This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained.…

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Title
Simple DNA Extraction Methods from Human Blood : A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and Chelex-100 for DNA Extraction from Human Blood Samples
Author
Samar Ali
Publisher
LAP LAMBERT Academic Publishing
Publication year
2014
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3659536342
ISBN 13
9783659536342
Item weight
102 grams
Dimensions
220x150x4 mm

AHA-BUCH GmbH

Einbeck, Germany

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