Restriction Endonucleases
Language: English
Published by Springer Berlin Heidelberg, Springer Berlin Heidelberg, 2004
- Hardcover
- New
Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH
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Seller Inventory # 9783540205029
- Title
- Restriction Endonucleases
- Author
- Alfred Pingoud
- Publisher
- Springer Berlin Heidelberg, Springer Berlin Heidelberg
- Publication year
- 2004
- Condition
- Neu
- Binding
- Buch
- Language
- English
- ISBN 10
- 3540205020
- ISBN 13
- 9783540205029
- Item weight
- 956 grams
- Dimensions
- 241x160x34 mm
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA.
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From the Back Cover
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
The present book deals with all aspects of restriction endonucleases including nomenclature, diversity, evolution, genetics, structure and function, mechanism of target site location and DNA recognition, enzymology, protein design, and provides a description of the history of the discovery of and the research on restriction enzymes.
"About the title" may belong to another edition of this title.
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