Quantitative PCR protocols. Methods in Molecular Medicine; 26.

Kochanowski, Bernd:

ISBN 10: 0896035182 / ISBN 13: 9780896035188

Published by Totowa, Humana Press, 1628

About this Item

316 S. Ehem. Bibliotheksexemplar mit Signatur und Stempel. GUTER Zustand, ein paar Gebrauchsspuren. Ex-library with stamp and library-signature. GOOD condition, some traces of use. R14990 9780896035188 Sprache: Englisch Gewicht in Gramm: 550.

Seller Inventory # 2471206

Bibliographic Details

• Title: Quantitative PCR protocols. Methods in Molecular Medicine; 26.
• Publisher: Totowa, Humana Press
• Publication Date: 1628
• Language: English
• ISBN 10: 0896035182
• ISBN 13: 9780896035188
• Binding: Hardcover
• Edition: 1st ed. 1999.
• Seller catalogs:
  • AA Varia

About this title

Synopsis

This is a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. With its step-by-step instructions, Quantitative PCR Protocols allows researchers to address biological and diagnostic questions that are difficult or impossible to answer using any other experimental approach.

From the Back Cover

In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs.
Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions.