Protein Engineering Protocols (Methods in Molecular Biology, 352)

ISBN 10: 1617373389 / ISBN 13: 9781617373381

Published by Humana, 2010

About this Item

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Bibliographic Details

• Title: Protein Engineering Protocols (Methods in Molecular Biology, 352)
• Publisher: Humana
• Publication Date: 2010
• Language: English
• ISBN 10: 1617373389
• ISBN 13: 9781617373381
• Binding: Soft cover
• Condition: Good

About this title

Synopsis

Protein Engineering Protocols considers the general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution. This volume provides a comprehensive guide to the methods used at every stage of the engineering process. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

From the Back Cover

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering.
The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented.
Following the successful Methods in Molecular Biology™ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators.