Platelets and Megakaryocytes: Volume 2: Perspectives and Techniques

Name: Platelets and Megakaryocytes: Volume 2: Perspectives and Techniques
Author: Gibbins, Jonathan M.|Mahaut-Smith, Martyn P.
ISBN: 9781617373053

Gibbins, Jonathan M.|Mahaut-Smith, Martyn P.

Language: English

Published by Humana Press, 2010

1617373052 / 9781617373053

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Includes supplementary material: sn.pub/extras12 The average human body has on the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in Western so.

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Title: Platelets and Megakaryocytes: Volume 2: Perspectives and Techniques
Author: Gibbins, Jonathan M.|Mahaut-Smith, Martyn P.
Publisher: Humana Press
Publication year: 2010
Condition: New
Binding: Soft cover
Language: English
ISBN 10: 1617373052
ISBN 13: 9781617373053

From the Back Cover

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

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