Plant Microtechnique and Microscopy (Paperback)

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Language: English

Published by Oxford University Press Inc, New York, 1999

0195089561 / 9780195089561

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Paperback. Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice ofmodern applications.The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditionalparaffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both textand diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to themicroscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory. This is a textbook and reference of methods in plant microtechnique. It includes traditional methods of preparing, sectioning, and staining materials, fluorescence cytochemistry and immunolocalization, molecular techniques of in situ hydridization, plastic techniques for light microscopy, and video and digital microscopy. This item is printed on demand. Shipping may be from multiple locations in the US or from the UK, depending on stock availability.…

Seller Inventory # 9780195089561

Title
Plant Microtechnique and Microscopy (Paperback)
Author
Editor
Publisher
Oxford University Press Inc, New York
Publication year
1999
Condition
new
Binding
Paperback
Language
English
ISBN 10
0195089561
ISBN 13
9780195089561

Grand Eagle Retail

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5-star seller

AbeBooks seller since October 12, 2005

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