PCR Protocols: Second Edition (Methods in Molecular Biology, Volume 226)

Bartlett, John M.; Stirling, David

ISBN 10: 0896036278 / ISBN 13: 9780896036277

Published by Humana Press, USA, 2003

About this Item

0896036278. Ring-bound softcover, from closed Astra Zeneca company library. Lending record shows this book has never been borrowed. 564 pages, index, illustrated with charts, tables and diagrams. A primer allowing the reader to gain basic experience of different PCR techniques, along with the more complex applications of PCR. An Essential reference for biochemists, biologists, geneticists and researchers utilizing the PCR techniques. The methods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. Contents, slight tanning around page edges, otherwise clean, and bright.

Seller Inventory # 030089

Bibliographic Details

• Title: PCR Protocols: Second Edition (Methods in Molecular Biology, Volume 226)
• Publisher: Humana Press, USA
• Publication Date: 2003
• Language: English
• ISBN 10: 0896036278
• ISBN 13: 9780896036277
• Binding: Ring Bound Softcover
• Edition: 2nd Edition
• Condition: Good
• Book Type: Book
• Seller catalogs:
  • biology

About this title

Synopsis

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.

From the Back Cover

Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications.