PCR for Detection of E. Coli from diarrhea patients

Language: English

Published by LAP LAMBERT Academic Publishing Apr 2015, 2015

3659706809 / 9783659706806

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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, GermanyBuchWeltWeit Ludwig Meier e.K.

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Softcover

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This item is printed on demand - it takes 3-4 days longer - Neuware -Bacterial diarrheal diseases represent one of the significant causes of human mortality and morbidity in developing countries that lack sufficient public health standards of sanitation. Escherichia coli is recognized to be the leading cause of diarrhea. The aim of present study was to detect bfp gene in stool samples of diarrheal patients by polymerase chain reaction in order to facilitate its diagnosis. In this study E. coli was detected in 87.5 % samples of diarrheal patients by production of characteristic colonies on MacConkey agar plates. On TSI slants 62.5 % diarrheal samples were identified as E. coli. It was found that 3.5 mM concentration yielded best results and 0.5 µg template was used as the optimum concentration required for specific amplification of bfp gene. 20 % of pure diarrheal E. coli strains showed positive results for bfp gene of Enteropathogenic E. coli with PCR assay. 56 pp. Englisch.

Seller Inventory # 9783659706806

Title
PCR for Detection of E. Coli from diarrhea patients
Author
Shaista Bashir
Publisher
LAP LAMBERT Academic Publishing Apr 2015
Publication year
2015
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3659706809
ISBN 13
9783659706806
Item weight
102 grams
Dimensions
220x150x4 mm

BuchWeltWeit Ludwig Meier e.K.

Bergisch Gladbach, Germany

5-star seller

AbeBooks seller since January 11, 2012

Shipping rates from Germany to U.S.A.

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BuchWeltWeit Ludwig Meier e.K.

Germany