Optimization of Agrobacterium Mediated Transformation in Tomato
Language: English
Published by LAP LAMBERT Academic Publishing Jun 2014, 2014
- Softcover
- New
Seller: buchversandmimpf2000, Emtmannsberg, BAYE, Germanybuchversandmimpf2000
AbeBooks seller since January 23, 2017
Condition: New
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This item is printed on demand - Print on Demand Titel. Neuware -Tomato is one of the most important vegetable crops throughout the world and is available in almost all seasons of the year in tropical and sub-tropical regions. Various conditions for the in vitro germination of tomato were optimized. When seeds were inoculated in half strength MS medium supplemented with 2mg/L BAP and 2mg/L of NAA reduced number of days was recorded for seed germination. A vector having reporter gene GUS was transformed into the four varieties of tomato cultivars. ViaAgrobacterium mediated transformation method. For all four cultivars two types of explants were used hypocotyl and cotyledonary leaves. After co cultivation and 7 days pre selection explants were subjected to the histochemical testing of GUS reporter gene. Theoptimized protocol for the Agrobacterium-mediated transformation of tomato was analyzed and comparered. Cotyledonary leaves and hypocotyl of four varieties of in vitro grown tomato was taken and used for Agrobacterium mediated transformation of vector Pzy102 containing GUS as reporter gene using strain EHA101. Many conditions were optimized for the efficient and optimized transformation of T-DNA into the tomato cultivars. Agrobacterium was grownVDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 100 pp. Englisch.…
Seller Inventory # 9783659540974
- Title
- Optimization of Agrobacterium Mediated Transformation in Tomato
- Author
- Faiza Samina
- Publisher
- LAP LAMBERT Academic Publishing Jun 2014
- Publication year
- 2014
- Condition
- Neu
- Binding
- Taschenbuch
- Language
- English
- ISBN 10
- 3659540978
- ISBN 13
- 9783659540974
- Item weight
- 167 grams
- Dimensions
- 220x150x7 mm
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buchversandmimpf2000
Emtmannsberg, BAYE, Germany
AbeBooks seller since January 23, 2017
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