Multiprotein Complexes

Language: English

Published by Humana Dez 2021, 2021

1071611283 / 9781071611289

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This item is printed on demand - Print on Demand Titel. Neuware -This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination¿x-ray crystallography and cryo-electron microscopy¿by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 352 pp. Englisch.…

Seller Inventory # 9781071611289

Title
Multiprotein Complexes
Author
Arnaud Poterszman
Publisher
Humana Dez 2021
Publication year
2021
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
1071611283
ISBN 13
9781071611289
Item weight
663 grams
Dimensions
254x178x20 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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