The Molecular Biology of Insect Disease Vectors | A Methods Manual

J. M. Crampton (u. a.)

ISBN 10: 0412736608 / ISBN 13: 9780412736605

Published by Springer Netherland, 1996

The Molecular Biology of Insect Disease Vectors | A Methods Manual | J. M. Crampton (u. a.) | Buch | xxv | Englisch | 1996 | Springer Netherland | EAN 9780412736605 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Seller Inventory # 102552632

Synopsis:

Only one generation ago, entomology was a proudly isolated discipline. In Comstock Hall, the building of the Department of Entomology at Cornell University where I was first introduced to experimental science in the laboratory of Tom Eisner, those of us interested in the chemistry of life felt like interlopers. In the 35 years that have elapsed since then, all of biology has changed, and entomology with it. Arrogant molecular biologists and resentful classical biologists might think that what has happened is a hostile take-over of biology by molecular biology. But they are wrong. More and more we now understand that the events were happier and much more exciting, amounting to a new synthesis. Molecular Biology, which was initially focused on the simplest of organisms, bacteria and viruses, broke out of its confines after the initial fundamental questions were answered - the structure of DNA, the genetic code, the nature of regulatory genes - and, importantly, as its methods became more and more generally applicable. The recombinant DNA revo­ lution of the 1970s, the development of techniques for sequencing macromolecules, the polymerase chain reaction, new molecular methods of genetic analysis, all brought molecular biology face to face with the infinite complexity and the exuber­ ant diversity of life. Molecular biology itself stopped being an isolated diScipline, pre­ occupied with the universal laws of life, and became an approach to addressing fas­ cinating specific problems from every field of biology.

Synopsis: The overall aim of this text is to provide a source book of detailed protocols covering the important techniques used in molecular entomology for those wishing to enter this field. The text is made up of 46 chapters, each covering a specific methodology and authored by an invited expert. The chapters are written such that a competent scientist who is unfamiliar with the method can carry out the technique successfully, either at the first attempt, or with a little practice, by simply following the detailed procedures. Each chapter includes: an introductory narrative; a detailed list of materials and equipment required; a detailed, step-by-step protocol; a section of explanatory notes; and a bibliography.The chapters are organised under section headings to provide a logical structure to the text, progressing from insect maintenance and infection with parasites, through basic molecular techniques, to genome mapping and transgenics. This methods manual will provide a valuable resource to assist in the development of this rapidly expanding field of research and scholarship.

Bibliographic Details

Title: The Molecular Biology of Insect Disease ...
Publisher: Springer Netherland
Publication Date: 1996
Binding: Buch
Condition: Neu