Microautoradiography and Electron Probe Analysis : Their Application to Plant Physiology

Language: English

Published by Springer, 1972

3540059504 / 9783540059509

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Seller: AHA-BUCH GmbH, Einbeck, GermanyAHA-BUCH GmbH

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Druck auf Anfrage Neuware - Printed after ordering - In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.…

Seller Inventory # 9783540059509

Title
Microautoradiography and Electron Probe Analysis : Their Application to Plant Physiology
Author
U. Lüttge
Publisher
Springer
Publication year
1972
Condition
Neu
Binding
Taschenbuch
Language
English
ISBN 10
3540059504
ISBN 13
9783540059509
Item weight
388 grams
Dimensions
235x155x14 mm

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